Improved production of isobutanol in pervaporation-coupled bioreactor using sugarcane bagasse hydrolysate in engineered Enterobacter aerogenes
DC Field | Value | Language |
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dc.contributor.author | Jung, Hwi-Min | - |
dc.contributor.author | Lee, Ju Yeon | - |
dc.contributor.author | Lee, Jung-Hyun | - |
dc.contributor.author | Oh, Min-Kyu | - |
dc.date.accessioned | 2021-09-02T09:51:01Z | - |
dc.date.available | 2021-09-02T09:51:01Z | - |
dc.date.created | 2021-06-16 | - |
dc.date.issued | 2018-07 | - |
dc.identifier.issn | 0960-8524 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/74832 | - |
dc.description.abstract | A process of isobutanol production from sugarcane bagasse hydrolysates in Enterobacter aerogenes was developed here with a pervaporation-integrated procedure. Isobutanol pathway was overexpressed in a mutant strain with eliminated byproduct-forming enzymes (LdhA, BudA, and PflB). A glucose-and-xylose-coconsuming ptsG mutant was constructed for effective utilization of lignocellulosic biomass. Toxic effects of isobutanol were alleviated by in situ recovery via a pervaporation procedure. Compared to single-batch fermentation, cell growth and isobutanol titer were improved by 60% and 100%, respectively, in the pervaporation-integrated fermentation process. A lab-made cross-linked polydimethylsiloxane membrane was cast on polyvinylidene fluoride and used in the pervaporation process. The membrane-penetrating condensate contained 55-226 gm(-2) h(-1) isobutanol with 6-25 g L-1 ethanol after separation. This study offers improved fermentative production of isobutanol from lignocellulosic biomass with a pervaporation procedure. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | ELSEVIER SCI LTD | - |
dc.subject | ACETONE-BUTANOL-ETHANOL | - |
dc.subject | ESCHERICHIA-COLI K-12 | - |
dc.subject | CHAIN HIGHER ALCOHOLS | - |
dc.subject | 2,3-BUTANEDIOL PRODUCTION | - |
dc.subject | HYDROGEN-PRODUCTION | - |
dc.subject | FURFURAL TOLERANCE | - |
dc.subject | BIO-ETHANOL | - |
dc.subject | N-BUTANOL | - |
dc.subject | FERMENTATION | - |
dc.subject | RECOVERY | - |
dc.title | Improved production of isobutanol in pervaporation-coupled bioreactor using sugarcane bagasse hydrolysate in engineered Enterobacter aerogenes | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Lee, Jung-Hyun | - |
dc.contributor.affiliatedAuthor | Oh, Min-Kyu | - |
dc.identifier.doi | 10.1016/j.biortech.2018.03.081 | - |
dc.identifier.scopusid | 2-s2.0-85044119210 | - |
dc.identifier.wosid | 000429820300048 | - |
dc.identifier.bibliographicCitation | BIORESOURCE TECHNOLOGY, v.259, pp.373 - 380 | - |
dc.relation.isPartOf | BIORESOURCE TECHNOLOGY | - |
dc.citation.title | BIORESOURCE TECHNOLOGY | - |
dc.citation.volume | 259 | - |
dc.citation.startPage | 373 | - |
dc.citation.endPage | 380 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Agriculture | - |
dc.relation.journalResearchArea | Biotechnology & Applied Microbiology | - |
dc.relation.journalResearchArea | Energy & Fuels | - |
dc.relation.journalWebOfScienceCategory | Agricultural Engineering | - |
dc.relation.journalWebOfScienceCategory | Biotechnology & Applied Microbiology | - |
dc.relation.journalWebOfScienceCategory | Energy & Fuels | - |
dc.subject.keywordPlus | ACETONE-BUTANOL-ETHANOL | - |
dc.subject.keywordPlus | ESCHERICHIA-COLI K-12 | - |
dc.subject.keywordPlus | CHAIN HIGHER ALCOHOLS | - |
dc.subject.keywordPlus | 2,3-BUTANEDIOL PRODUCTION | - |
dc.subject.keywordPlus | HYDROGEN-PRODUCTION | - |
dc.subject.keywordPlus | FURFURAL TOLERANCE | - |
dc.subject.keywordPlus | BIO-ETHANOL | - |
dc.subject.keywordPlus | N-BUTANOL | - |
dc.subject.keywordPlus | FERMENTATION | - |
dc.subject.keywordPlus | RECOVERY | - |
dc.subject.keywordAuthor | Enterobacter aerogenes | - |
dc.subject.keywordAuthor | Isobutanol | - |
dc.subject.keywordAuthor | Pervaporation | - |
dc.subject.keywordAuthor | Lignocellulosic biomass | - |
dc.subject.keywordAuthor | Sugarcane bagasse hydrolysate | - |
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