Improved production of isobutanol in pervaporation-coupled bioreactor using sugarcane bagasse hydrolysate in engineered Enterobacter aerogenes
- Authors
- Jung, Hwi-Min; Lee, Ju Yeon; Lee, Jung-Hyun; Oh, Min-Kyu
- Issue Date
- 7월-2018
- Publisher
- ELSEVIER SCI LTD
- Keywords
- Enterobacter aerogenes; Isobutanol; Pervaporation; Lignocellulosic biomass; Sugarcane bagasse hydrolysate
- Citation
- BIORESOURCE TECHNOLOGY, v.259, pp.373 - 380
- Indexed
- SCIE
SCOPUS
- Journal Title
- BIORESOURCE TECHNOLOGY
- Volume
- 259
- Start Page
- 373
- End Page
- 380
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/74832
- DOI
- 10.1016/j.biortech.2018.03.081
- ISSN
- 0960-8524
- Abstract
- A process of isobutanol production from sugarcane bagasse hydrolysates in Enterobacter aerogenes was developed here with a pervaporation-integrated procedure. Isobutanol pathway was overexpressed in a mutant strain with eliminated byproduct-forming enzymes (LdhA, BudA, and PflB). A glucose-and-xylose-coconsuming ptsG mutant was constructed for effective utilization of lignocellulosic biomass. Toxic effects of isobutanol were alleviated by in situ recovery via a pervaporation procedure. Compared to single-batch fermentation, cell growth and isobutanol titer were improved by 60% and 100%, respectively, in the pervaporation-integrated fermentation process. A lab-made cross-linked polydimethylsiloxane membrane was cast on polyvinylidene fluoride and used in the pervaporation process. The membrane-penetrating condensate contained 55-226 gm(-2) h(-1) isobutanol with 6-25 g L-1 ethanol after separation. This study offers improved fermentative production of isobutanol from lignocellulosic biomass with a pervaporation procedure.
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Collections - College of Engineering > Department of Chemical and Biological Engineering > 1. Journal Articles
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