High-yield production of 1,3-propanediol from glycerol by metabolically engineered Klebsiella pneumoniae
DC Field | Value | Language |
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dc.contributor.author | Lee, Jung Hun | - |
dc.contributor.author | Jung, Moo-Young | - |
dc.contributor.author | Oh, Min-Kyu | - |
dc.date.accessioned | 2021-09-02T12:43:20Z | - |
dc.date.available | 2021-09-02T12:43:20Z | - |
dc.date.created | 2021-06-16 | - |
dc.date.issued | 2018-04-09 | - |
dc.identifier.issn | 1754-6834 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/76152 | - |
dc.description.abstract | Background: Glycerol is a major byproduct of the biodiesel industry and can be converted to 1,3-propanediol (1,3-PDO) by microorganisms through a two-step enzymatic reaction. The production of 1,3-PDO from glycerol using microorganisms is accompanied by formation of unwanted byproducts, including lactate and 2,3-butanediol, resulting in a low-conversion yield. Results: Klebsiella pneumoniae was metabolically engineered to produce high-molar yield of 1,3-PDO from glycerol. First, the pathway genes for byproduct formation were deleted in K. pneumoniae. Then, glycerol assimilation pathways were eliminated and mannitol was co-fed to the medium. Finally, transcriptional regulation of the dha operon were genetically modified for enhancing 1,3-propanediol production. The batch fermentation of the engineered strain with co-feeding of a small amount of mannitol yielded 0.76 mol 1,3-PDO from 1 mol glycerol. Conclusions: Klebsiella pneumoniae is useful microorganism for producing 1,3-PDO from glycerol. Implemented engineering in this study successfully improved 1,3-PDO production yield, which is significantly higher than those reported in previous studies. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | BIOMED CENTRAL LTD | - |
dc.subject | ESCHERICHIA-COLI K-12 | - |
dc.subject | GENE-EXPRESSION | - |
dc.subject | GENOME SEQUENCE | - |
dc.subject | DHA REGULON | - |
dc.subject | 2,3-BUTANEDIOL | - |
dc.subject | OVEREXPRESSION | - |
dc.subject | BIOSYNTHESIS | - |
dc.subject | FERMENTATION | - |
dc.subject | BACTERIA | - |
dc.subject | DELETION | - |
dc.title | High-yield production of 1,3-propanediol from glycerol by metabolically engineered Klebsiella pneumoniae | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Oh, Min-Kyu | - |
dc.identifier.doi | 10.1186/s13068-018-1100-5 | - |
dc.identifier.scopusid | 2-s2.0-85045267392 | - |
dc.identifier.wosid | 000429434600001 | - |
dc.identifier.bibliographicCitation | BIOTECHNOLOGY FOR BIOFUELS, v.11 | - |
dc.relation.isPartOf | BIOTECHNOLOGY FOR BIOFUELS | - |
dc.citation.title | BIOTECHNOLOGY FOR BIOFUELS | - |
dc.citation.volume | 11 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biotechnology & Applied Microbiology | - |
dc.relation.journalResearchArea | Energy & Fuels | - |
dc.relation.journalWebOfScienceCategory | Biotechnology & Applied Microbiology | - |
dc.relation.journalWebOfScienceCategory | Energy & Fuels | - |
dc.subject.keywordPlus | ESCHERICHIA-COLI K-12 | - |
dc.subject.keywordPlus | GENE-EXPRESSION | - |
dc.subject.keywordPlus | GENOME SEQUENCE | - |
dc.subject.keywordPlus | DHA REGULON | - |
dc.subject.keywordPlus | 2,3-BUTANEDIOL | - |
dc.subject.keywordPlus | OVEREXPRESSION | - |
dc.subject.keywordPlus | BIOSYNTHESIS | - |
dc.subject.keywordPlus | FERMENTATION | - |
dc.subject.keywordPlus | BACTERIA | - |
dc.subject.keywordPlus | DELETION | - |
dc.subject.keywordAuthor | 1, 3-PDO | - |
dc.subject.keywordAuthor | Klebsiella pneumoniae | - |
dc.subject.keywordAuthor | Glycerol | - |
dc.subject.keywordAuthor | Byproducts | - |
dc.subject.keywordAuthor | Glycerol assimilation pathway | - |
dc.subject.keywordAuthor | Co-substrate | - |
dc.subject.keywordAuthor | Mannitol | - |
dc.subject.keywordAuthor | dha operon | - |
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