High-yield production of 1,3-propanediol from glycerol by metabolically engineered Klebsiella pneumoniae
- Authors
- Lee, Jung Hun; Jung, Moo-Young; Oh, Min-Kyu
- Issue Date
- 9-4월-2018
- Publisher
- BIOMED CENTRAL LTD
- Keywords
- 1, 3-PDO; Klebsiella pneumoniae; Glycerol; Byproducts; Glycerol assimilation pathway; Co-substrate; Mannitol; dha operon
- Citation
- BIOTECHNOLOGY FOR BIOFUELS, v.11
- Indexed
- SCIE
SCOPUS
- Journal Title
- BIOTECHNOLOGY FOR BIOFUELS
- Volume
- 11
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/76152
- DOI
- 10.1186/s13068-018-1100-5
- ISSN
- 1754-6834
- Abstract
- Background: Glycerol is a major byproduct of the biodiesel industry and can be converted to 1,3-propanediol (1,3-PDO) by microorganisms through a two-step enzymatic reaction. The production of 1,3-PDO from glycerol using microorganisms is accompanied by formation of unwanted byproducts, including lactate and 2,3-butanediol, resulting in a low-conversion yield. Results: Klebsiella pneumoniae was metabolically engineered to produce high-molar yield of 1,3-PDO from glycerol. First, the pathway genes for byproduct formation were deleted in K. pneumoniae. Then, glycerol assimilation pathways were eliminated and mannitol was co-fed to the medium. Finally, transcriptional regulation of the dha operon were genetically modified for enhancing 1,3-propanediol production. The batch fermentation of the engineered strain with co-feeding of a small amount of mannitol yielded 0.76 mol 1,3-PDO from 1 mol glycerol. Conclusions: Klebsiella pneumoniae is useful microorganism for producing 1,3-PDO from glycerol. Implemented engineering in this study successfully improved 1,3-PDO production yield, which is significantly higher than those reported in previous studies.
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Collections - College of Engineering > Department of Chemical and Biological Engineering > 1. Journal Articles
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