In-situ and highly sensitive detection of epidermal growth factor receptor mutation using nano-porous quartz crystal microbalance
- Authors
- Park, Hyunjun; You, Juneseok; Park, Chanho; Jang, Kuewhan; Na, Sungsoo
- Issue Date
- 4월-2018
- Publisher
- KOREAN SOC MECHANICAL ENGINEERS
- Keywords
- Epidermal growth factor receptor (EGFR); In-situ detection; Mutation; Nano-porous; Quartz crystal microbalance (QCM)
- Citation
- JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY, v.32, no.4, pp.1927 - 1932
- Indexed
- SCIE
SCOPUS
KCI
- Journal Title
- JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY
- Volume
- 32
- Number
- 4
- Start Page
- 1927
- End Page
- 1932
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/76606
- DOI
- 10.1007/s12206-018-0348-9
- ISSN
- 1738-494X
- Abstract
- Assessment of epidermal growth factor receptor (EGFR) exon 19 mutations is very important indicator for treating lung cancer patients. In this report, we propose in-situ and highly sensitive detection method of EGFR mutation using quartz crystal microbalance (QCM) and nano-porous structured QCM electrode. The detection is based on the resonance frequency shift of QCM electrode upon DNA hybridization between probe DNA and EGFR mutant DNA (746-750 (del ELREA) in EGFR exon 19). Nano-porous structure is produced on the QCM electrode through simple processes of electrochemical deposition and etching. By using QCM, in-situ detection of EGFR mutation is performed within 30 mins. High sensitivity is achieved by introducing nano-porous structure on a QCM electrode, and the limit of detection (LOD) is 1 nM.
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Collections - College of Engineering > Department of Mechanical Engineering > 1. Journal Articles
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