A Fluorescent Probe for Stimulated Emission Depletion Super-Resolution Imaging of Vicinal-Dithiol-Proteins on Mitochondria! Membrane
- Authors
- Yang, Zhigang; Kang, Dong Hoon; Lee, Hoyeon; Shin, Jinwoo; Yan, Wei; Rathore, Bhowmira; Kim, Hye-Ri; Kim, Seo Jin; Singh, Hardev; Liu, Liwei; Qu, Junle; Kang, Chulhun; Kim, Jong Seung
- Issue Date
- 4월-2018
- Publisher
- AMER CHEMICAL SOC
- Citation
- BIOCONJUGATE CHEMISTRY, v.29, no.4, pp.1446 - 1453
- Indexed
- SCIE
SCOPUS
- Journal Title
- BIOCONJUGATE CHEMISTRY
- Volume
- 29
- Number
- 4
- Start Page
- 1446
- End Page
- 1453
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/76633
- DOI
- 10.1021/acs.bioconjchem.8b00128
- ISSN
- 1043-1802
- Abstract
- Realizing the significant roles of vicinal-dithiol proteins (VDPs) in maintaining the cellular redox homeostasis and their implication in many diseases, we synthesized a smart arsenate based fluorescent probe 1 which can preferentially target the mitochondrial membrane-bound vicinal dithiol proteins (VDPs), especially voltage-dependent anion channel (VDAC2). The probe targetability was demonstrated by in vitro studies such as colocalization, stimulated emission depletion (STED) super-resolution imaging, proteomic MS/MS analysis, and Western blot analysis. The probe represents a rare example of fluorescence labeling of mitochondrial membrane-bound VDPs and can provide a new way to construct VDPs-specific fluorescent probes to gain deeper understanding of their roles in mitochondrial-related disorders.
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- Appears in
Collections - College of Science > Department of Chemistry > 1. Journal Articles
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