Label-free and live cell imaging by interferometric scattering microscopy
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Park, Jin-Sung | - |
dc.contributor.author | Lee, Il-Buem | - |
dc.contributor.author | Moon, Hyeon-Min | - |
dc.contributor.author | Joo, Jong-Hyeon | - |
dc.contributor.author | Kim, Kyoung-Hoon | - |
dc.contributor.author | Hong, Seok-Cheol | - |
dc.contributor.author | Cho, Minhaeng | - |
dc.date.accessioned | 2021-09-02T13:47:09Z | - |
dc.date.available | 2021-09-02T13:47:09Z | - |
dc.date.created | 2021-06-16 | - |
dc.date.issued | 2018-03-14 | - |
dc.identifier.issn | 2041-6520 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/76731 | - |
dc.description.abstract | Despite recent remarkable advances in microscopic techniques, it still remains very challenging to directly observe the complex structure of cytoplasmic organelles in live cells without a fluorescent label. Here we report label-free and live-cell imaging of mammalian cell, Escherischia coli, and yeast, using interferometric scattering microscopy, which reveals the underlying structures of a variety of cytoplasmic organelles as well as the underside structure of the cells. The contact areas of the cells attached onto a glass substrate, e.g., focal adhesions and filopodia, are clearly discernible. We also found a variety of fringe-like features in the cytoplasmic area, which may reflect the folded structures of cytoplasmic organelles. We thus anticipate that the label-free interferometric scattering microscopy can be used as a powerful tool to shed interferometric light on in vivo structures and dynamics of various intracellular phenomena. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | ROYAL SOC CHEMISTRY | - |
dc.subject | COHERENT BRIGHTFIELD MICROSCOPY | - |
dc.subject | SINGLE-MOLECULE LEVEL | - |
dc.subject | REFRACTIVE-INDEX | - |
dc.subject | GENERATION MICROSCOPY | - |
dc.subject | CARS MICROSCOPY | - |
dc.subject | LIVING CELLS | - |
dc.subject | TRACKING | - |
dc.subject | SPEED | - |
dc.subject | MITOCHONDRIA | - |
dc.subject | DYNAMICS | - |
dc.title | Label-free and live cell imaging by interferometric scattering microscopy | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Hong, Seok-Cheol | - |
dc.contributor.affiliatedAuthor | Cho, Minhaeng | - |
dc.identifier.doi | 10.1039/c7sc04733a | - |
dc.identifier.scopusid | 2-s2.0-85043481891 | - |
dc.identifier.wosid | 000431100300005 | - |
dc.identifier.bibliographicCitation | CHEMICAL SCIENCE, v.9, no.10, pp.2690 - 2697 | - |
dc.relation.isPartOf | CHEMICAL SCIENCE | - |
dc.citation.title | CHEMICAL SCIENCE | - |
dc.citation.volume | 9 | - |
dc.citation.number | 10 | - |
dc.citation.startPage | 2690 | - |
dc.citation.endPage | 2697 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Multidisciplinary | - |
dc.subject.keywordPlus | COHERENT BRIGHTFIELD MICROSCOPY | - |
dc.subject.keywordPlus | SINGLE-MOLECULE LEVEL | - |
dc.subject.keywordPlus | REFRACTIVE-INDEX | - |
dc.subject.keywordPlus | GENERATION MICROSCOPY | - |
dc.subject.keywordPlus | CARS MICROSCOPY | - |
dc.subject.keywordPlus | LIVING CELLS | - |
dc.subject.keywordPlus | TRACKING | - |
dc.subject.keywordPlus | SPEED | - |
dc.subject.keywordPlus | MITOCHONDRIA | - |
dc.subject.keywordPlus | DYNAMICS | - |
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