Label-free and live cell imaging by interferometric scattering microscopy
- Authors
- Park, Jin-Sung; Lee, Il-Buem; Moon, Hyeon-Min; Joo, Jong-Hyeon; Kim, Kyoung-Hoon; Hong, Seok-Cheol; Cho, Minhaeng
- Issue Date
- 14-3월-2018
- Publisher
- ROYAL SOC CHEMISTRY
- Citation
- CHEMICAL SCIENCE, v.9, no.10, pp.2690 - 2697
- Indexed
- SCIE
SCOPUS
- Journal Title
- CHEMICAL SCIENCE
- Volume
- 9
- Number
- 10
- Start Page
- 2690
- End Page
- 2697
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/76731
- DOI
- 10.1039/c7sc04733a
- ISSN
- 2041-6520
- Abstract
- Despite recent remarkable advances in microscopic techniques, it still remains very challenging to directly observe the complex structure of cytoplasmic organelles in live cells without a fluorescent label. Here we report label-free and live-cell imaging of mammalian cell, Escherischia coli, and yeast, using interferometric scattering microscopy, which reveals the underlying structures of a variety of cytoplasmic organelles as well as the underside structure of the cells. The contact areas of the cells attached onto a glass substrate, e.g., focal adhesions and filopodia, are clearly discernible. We also found a variety of fringe-like features in the cytoplasmic area, which may reflect the folded structures of cytoplasmic organelles. We thus anticipate that the label-free interferometric scattering microscopy can be used as a powerful tool to shed interferometric light on in vivo structures and dynamics of various intracellular phenomena.
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Collections - College of Science > Department of Physics > 1. Journal Articles
- College of Science > Department of Chemistry > 1. Journal Articles
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