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Synthesis of Fatty Acid Methyl Esters Using Mixed Enzyme in a Packed Bed Reactor

Authors
Ryu, JiinChoi, NakyungKim, HeejinKim, Byung HeeKim, Hak-RyulKim, In-Hwan
Issue Date
3월-2018
Publisher
JAPAN OIL CHEMISTS SOC
Keywords
fatty acid methyl ester; mixed enzyme; packed bed reactor; palm fatty acid distillate; esterification
Citation
JOURNAL OF OLEO SCIENCE, v.67, no.3, pp.321 - 326
Indexed
SCIE
SCOPUS
Journal Title
JOURNAL OF OLEO SCIENCE
Volume
67
Number
3
Start Page
321
End Page
326
URI
https://scholar.korea.ac.kr/handle/2021.sw.korea/77230
DOI
10.5650/jos.ess17198
ISSN
1345-8957
Abstract
Fatty acid methyl esters were synthesized from palm fatty acid distillate (PFAD) and methanol in a packed bed reactor via lipase-catalyzed esterification. The PFAD consisted of 91 wt% of free fatty acids, 2 wt% monoacylglycerides, 3 wt% diacylglycerides, and 4 wt% triacylglycerides. t-Butanol was employed as a reaction medium and a mixed enzyme consisting of Lipozyme TL IM from Thermomyces lanuginosus and Novozym 435 from Candida antarctica was employed as the biocatalyst. The effect of mixed enzyme was investigated and the optimum blending ratio (w/w) of Novozym 435 to Lipozyme TL IM was 5:95. Using the mixed enzyme, the optimum molar ratio (PFAD to methanol) and temperature were determined to be 1:6 and 30 degrees C, respectively. Under the optimized conditions, the maximum yield of ca. 96% was achieved.
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Kim, In Hwan
보건과학대학 (바이오시스템의과학부)
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