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Nelumbo nucifera Seed Extract Promotes Sleep in Drosophila melanogaster

Authors
Jo, KyungaeChoi, Hyeon-SonJeon, SangDuckAhn, Chang-WonSuh, Hyung Joo
Issue Date
3월-2018
Publisher
PHARMACEUTICAL SOC JAPAN
Keywords
Nelumbo nucifera; insomnia; sleep; caffeine; gamma-aminobutyric acid; Drosophila melanogaster
Citation
BIOLOGICAL & PHARMACEUTICAL BULLETIN, v.41, no.3, pp.399 - 408
Indexed
SCIE
SCOPUS
Journal Title
BIOLOGICAL & PHARMACEUTICAL BULLETIN
Volume
41
Number
3
Start Page
399
End Page
408
URI
https://scholar.korea.ac.kr/handle/2021.sw.korea/77269
ISSN
0918-6158
Abstract
The sleep-promoting effects of the water extract of Nelumbo nucifera seeds (NNE) were investigated in an invertebrate model. The effects of NNE on the subjective nighttime activity, sleep episodes, and sleep time were determined using Drosophila melanogaster and locomotor activity monitoring systems in basal and caffeine-induced arousal conditions. The movements of fruit flies were analyzed using the Noldus EthoVision-XT system, and the levels of neuromodulators were analyzed using HPLC. Expression of neuromodulator receptors was analyzed using real-time PCR. NNE was shown to contain neurotransmission-related components; gamma-aminobutyric acid (GABA) (2.33 +/- 0.22 mg/g), tryptophan (2.00 +/- 0.06 mg/g), quinidine (0.55 +/- 0.33 mg/g), and neferine (0.16 +/- 0.01 mg/g). The total activity of flies during nighttime was decreased by 52% with 1.0% NNE treatment. In the individual and collective conditions, the subjective nighttime activities (45/38%) and sleep bouts (20/14%) of flies was significantly decreased with NNE treatment, while total sleep times (10/27%) were significantly increased. This sleep-promoting effect is more pronounced in caffeine-treated conditions; the nighttime activity of flies was reduced by 53%, but total sleep time was increased by 60%. Our video tracking analysis showed a significant decrease of the moving distance and velocity of flies by NNE. This NNE-mediated sleep-promoting effect was associated with up-regulation of GABAA/GABAB and serotonin receptors. The NNE-mediated increase of GABA content was identified in flies. These results demonstrate that NNE effectively promotes sleep in flies by regulating the GABAergic/serotonergic neuromodulators, and could be an alternative agent for sleep promotion.
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Suh, Hyung Joo
보건과학대학 (바이오시스템의과학부)
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