Conversion of glioma cells to glioma stem-like cells by angiocrine factors
- Authors
- Kim, Jun-Kyum; Jeon, Hye-Min; Jeon, Hee-Young; Oh, Se-Yeong; Kim, Eun-Jung; Jin, Xiong; Kim, Se-Hoon; Kim, Sung-Hak; Jin, Xun; Kim, Hyunggee
- Issue Date
- 19-Feb-2018
- Publisher
- ACADEMIC PRESS INC ELSEVIER SCIENCE
- Keywords
- Angiocrine factors; Glioma cells; Glioma stem-like cells; ID4; OCT4
- Citation
- BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, v.496, no.4, pp.1013 - 1018
- Indexed
- SCIE
SCOPUS
- Journal Title
- BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
- Volume
- 496
- Number
- 4
- Start Page
- 1013
- End Page
- 1018
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/77333
- DOI
- 10.1016/j.bbrc.2017.02.076
- ISSN
- 0006-291X
- Abstract
- Glioma stem-like cells (GSCs) contribute to tumor initiation, progression, and therapeutic resistance, but their cellular origin remains largely unknown. Here, using a stem/progenitor cell-fate tracking reporter system in which eGFP is expressed by promoter of OCT4 that is activated in stem/progenitor cells, we demonstrate that eGFP-negative glioma cells (GCs) became eGFP-positive-GCs in both in vitro cultures and in vivo xenografts. These eGFP-positive-GCs exhibited GSC features and primarily localized to the perivascular region in tumor xenografts, similar to the existence of OCT4-expressing GCs in the perivascular region of human glioblastoma specimens. Angiocrine factors, including nitric oxide (NO), converted eGFP-negative-GCs into eGFP-positive-GCs. Mechanistically, NO signaling conferred GSC features to GCs by increasing OCT4 and NOTCH signaling via ID4. NO signaling blockade and a suicide gene induction prevented tumorigenicity with a decrease in eGFP-positive-GCs in the perivascular region. Taken together, our results reveal the molecular mechanism underlying GSCs generation by cancer cell dedifferentiation. (C) 2017 Elsevier Inc. All rights reserved.
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Collections - Graduate School > Department of Biotechnology > 1. Journal Articles
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