Terminal Deoxynucleotidyl Transferase-Mediated Deoxyuridine Triphosphate Nick End Labeling (TUNEL) Assay to Characterize Histopathologic Changes Following Thermal Injury
- Authors
- Lee, Ji Min; Park, Ji Hyun; Kim, Bo Young; Kim, Il-Hwan
- Issue Date
- 2월-2018
- Publisher
- KOREAN DERMATOLOGICAL ASSOC
- Keywords
- Carbon dioxide lasers; In situ nick-end labeling; Neodymium-doped yttrium aluminum garnet lasers; Radiofrequency; Thermal destruction
- Citation
- ANNALS OF DERMATOLOGY, v.30, no.1, pp.41 - 46
- Indexed
- SCIE
SCOPUS
KCI
- Journal Title
- ANNALS OF DERMATOLOGY
- Volume
- 30
- Number
- 1
- Start Page
- 41
- End Page
- 46
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/77411
- DOI
- 10.5021/ad.2018.30.1.41
- ISSN
- 1013-9087
- Abstract
- Background: Despite the wide application of lasers and radiofrequency (RF) surgery in dermatology, it is difficult to find studies showing the extent of damage dependent on cell death. Objective: We evaluated histopathologic changes following in vivo thermal damage generated by CO2 laser, 1,444 nm long-pulsed neodymium:yttrium-aluminum-garnet (LP Nd:YAG) laser and RF emitting electrosurgical unit. Methods: Thermal damage was induced by the above instruments on ventral skin of rat. Specimens were stained with hematoxylin and eosin, along with a terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate nick end labeling (TUNEL) assay, to highlight the degree of irreversible cellular injury. Results: The volume of vaporization was largest with the CO2 laser. Area of cell death area identified by TUNEL assay, when arranged from widest to narrowest, was 1,444 nm LP Nd:YAG laser, CO2 laser, and RF emitting electrosurgical unit. Conclusion: This histopathologic evaluation of the acute characterization of injury across devices may be advantageous for attaining better treatment outcomes.
- Files in This Item
- There are no files associated with this item.
- Appears in
Collections - College of Medicine > Department of Medical Science > 1. Journal Articles
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.