Electropreconcentration-induced local pH change
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Chun, Honggu | - |
dc.date.accessioned | 2021-09-02T15:16:14Z | - |
dc.date.available | 2021-09-02T15:16:14Z | - |
dc.date.created | 2021-06-16 | - |
dc.date.issued | 2018-02 | - |
dc.identifier.issn | 0173-0835 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/77489 | - |
dc.description.abstract | Ion-permselective nanochannel-based sample preconcentration or electropreconcentration has been demonstrated as an effective technique for concentrating charged analytes at the interface between a micro- and nanochannel. The anion-selective electropreconcentration involves extraction of hydroxide in the preconcentrated sample plug, resulting in pH decrease. We investigated the pH change in a microchannel using charged pH indicators with different conditions including running buffer pH, sample channel electric field, and salt concentration. The anion-selective preconcentration showed pH decrease from 11 to under 7 in the preconcentrated sample plug. Therefore, careful design and interpretation are required with pH-dependent experiments such as analyzing enzyme or antibody characteristics. The pH change could be mitigated by reducing the sample channel electric field and/or increasing salt concentration in the buffer. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | WILEY | - |
dc.subject | SAMPLE PRECONCENTRATION | - |
dc.subject | CONCENTRATION POLARIZATION | - |
dc.subject | MICROFLUIDIC PLATFORM | - |
dc.subject | ASSAY | - |
dc.subject | ELECTROPHORESIS | - |
dc.subject | CHARGE | - |
dc.subject | SENSITIVITY | - |
dc.subject | SEPARATION | - |
dc.subject | PROTEINS | - |
dc.title | Electropreconcentration-induced local pH change | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Chun, Honggu | - |
dc.identifier.doi | 10.1002/elps.201700373 | - |
dc.identifier.scopusid | 2-s2.0-85034957553 | - |
dc.identifier.wosid | 000424154000012 | - |
dc.identifier.bibliographicCitation | ELECTROPHORESIS, v.39, no.3, pp.521 - 525 | - |
dc.relation.isPartOf | ELECTROPHORESIS | - |
dc.citation.title | ELECTROPHORESIS | - |
dc.citation.volume | 39 | - |
dc.citation.number | 3 | - |
dc.citation.startPage | 521 | - |
dc.citation.endPage | 525 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.relation.journalWebOfScienceCategory | Biochemical Research Methods | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Analytical | - |
dc.subject.keywordPlus | SAMPLE PRECONCENTRATION | - |
dc.subject.keywordPlus | CONCENTRATION POLARIZATION | - |
dc.subject.keywordPlus | MICROFLUIDIC PLATFORM | - |
dc.subject.keywordPlus | ASSAY | - |
dc.subject.keywordPlus | ELECTROPHORESIS | - |
dc.subject.keywordPlus | CHARGE | - |
dc.subject.keywordPlus | SENSITIVITY | - |
dc.subject.keywordPlus | SEPARATION | - |
dc.subject.keywordPlus | PROTEINS | - |
dc.subject.keywordAuthor | Electropreconcentration | - |
dc.subject.keywordAuthor | Local pH change | - |
dc.subject.keywordAuthor | Sample preconcentration | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
(02841) 서울특별시 성북구 안암로 14502-3290-1114
COPYRIGHT © 2021 Korea University. All Rights Reserved.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.