Electropreconcentration-induced local pH change
- Authors
- Chun, Honggu
- Issue Date
- 2월-2018
- Publisher
- WILEY
- Keywords
- Electropreconcentration; Local pH change; Sample preconcentration
- Citation
- ELECTROPHORESIS, v.39, no.3, pp.521 - 525
- Indexed
- SCIE
SCOPUS
- Journal Title
- ELECTROPHORESIS
- Volume
- 39
- Number
- 3
- Start Page
- 521
- End Page
- 525
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/77489
- DOI
- 10.1002/elps.201700373
- ISSN
- 0173-0835
- Abstract
- Ion-permselective nanochannel-based sample preconcentration or electropreconcentration has been demonstrated as an effective technique for concentrating charged analytes at the interface between a micro- and nanochannel. The anion-selective electropreconcentration involves extraction of hydroxide in the preconcentrated sample plug, resulting in pH decrease. We investigated the pH change in a microchannel using charged pH indicators with different conditions including running buffer pH, sample channel electric field, and salt concentration. The anion-selective preconcentration showed pH decrease from 11 to under 7 in the preconcentrated sample plug. Therefore, careful design and interpretation are required with pH-dependent experiments such as analyzing enzyme or antibody characteristics. The pH change could be mitigated by reducing the sample channel electric field and/or increasing salt concentration in the buffer.
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Collections - Graduate School > Department of Bioengineering > 1. Journal Articles
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