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An artificial synthetic pathway for acetoin, 2,3-butanediol, and 2-butanol production from ethanol using cell free multi-enzyme catalysis
- Authors
- Zhang, Liaoyuan; Singh, Raushan; Sivakumar, D.; Guo, Zewang; Li, Jiahuan; Chen, Fanbing; He, Yuanzhi; Guan, Xiong; Kang, Yun Chan; Lee, Jung-Kul
- Issue Date
- 7-Jan-2018
- Publisher
- ROYAL SOC CHEMISTRY
- Citation
- GREEN CHEMISTRY, v.20, no.1, pp.230 - 242
- Indexed
- SCIE
SCOPUS
- Journal Title
- GREEN CHEMISTRY
- Volume
- 20
- Number
- 1
- Start Page
- 230
- End Page
- 242
- ISSN
- 1463-9262
- Abstract
- Upgrading ethanol to higher order alcohols is desired but difficult using current biotechnological methods. In this study, we designed a completely artificial reaction pathway for upgrading ethanol to acetoin, 2,3-butanediol, and 2-butanol in a cell-free bio-system composed of ethanol dehydrogenase, formolase, 2,3-butanediol dehydrogenase, diol dehydratase, and NADH oxidase. Under optimized conditions, acetoin, 2,3-butanediol, and 2-butanol were produced at 88.78%, 88.28%, and 27.25% of the theoretical yield from 100 mM ethanol, respectively. These results demonstrate that this artificial synthetic pathway is an environmentally-friendly novel approach for upgrading bio-ethanol to acetoin, 2,3-butanediol, and 2-butanol.
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