Demineralized Dentin Matrix as a Carrier of Recombinant Human Bone Morphogenetic Proteins: in Vivo Study
- Authors
- Um, In-Woong; Kim, Young-Kyun; Jun, Sang-Ho; Kim, Moon-Young; Cui, Nianhui
- Issue Date
- 2018
- Publisher
- JOURNAL HARD TISSUE BIOLOGY
- Keywords
- Bone formation; Demineralized dentin matrix (DDM); Recombinant human bone morphogenetic protein-2 (rhBMP-2); rhBMP-2 carrier
- Citation
- JOURNAL OF HARD TISSUE BIOLOGY, v.27, no.3, pp.219 - 226
- Indexed
- SCIE
SCOPUS
- Journal Title
- JOURNAL OF HARD TISSUE BIOLOGY
- Volume
- 27
- Number
- 3
- Start Page
- 219
- End Page
- 226
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/80869
- DOI
- 10.2485/jhtb.27.219
- ISSN
- 1341-7649
- Abstract
- This study aimed to evaluate the efficacy of rabbit demineralized dentin matrix (DDM) as a recombinant human bone morphogenetic protein-2 (rhBMP-2) carrier using the subcutaneous tissues of mice and rabbit calvarial critical-sized defects. DDM of rabbit, combined with rhBMP-2 (DDM/rhBMP-2) was transplanted into the subcutaneous tissues of 6 mice and 6 rabbit calvarial critical-sized defects (DDM = 0.03 g, control; DDM/rhBMP-2 = 0.03 g of DDM, 0.2 mg/ml, 5.0 mu g of rhBMP-2, experimental). Both DDM and DDM/rhBMP-2 was transplanted into the left and right subcutaneous tissues of mice symmetrically. For rabbits, 4 round critical-sized defects (8 mm diameter) were formed on the exposed skull. DDM was transplanted into the 2 defects on the left sides (n = 12) and DDM/rhBMP-2 into the right sides (n = 12). Two animals among 6 mice and 6 rabbits were sacrificed respectively at the 1, 2, and 4 experimental weeks for the histological and histomorphometrical evaluations with hematoxylin and eosin staining. Tissues from rabbits were imaged via micro-computed tomography (mu CT). DDM/rhBMP-2 in mice induced new bone formation at 2 weeks and maturation with bone marrow at 4 weeks. DDM/rhBMP-2 in rabbit calvarium induced new bone formation remarkably at 4 weeks 21.77-47.99% compared to the DDM. These observations suggest that DDM could be considered a potential carrier of rhBMP-2. The rhBMP-2 loaded on DDM enhanced bone formation.
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