Generation of induced pluripotent stem cell (iPSC) line from a 21-year-old X-linked adrenoleukodystrophy (X-ALD) patient

Abstract

X-linked Adrenoleukodystrophy (X-ALD) is a genetic disease that caused bymutations in adenosine triphosphate [ ATP]-binding-cassette transporter superfamily D member 1 (ABCD1) gene. We generated an induced pluripotent stem cell (iPSC) line from a 21-year-old male X-ALD patient-derived fibroblasts by Sendai virus mediated reprogramming. Established iPSCs stably expanded while maintaining immunoreactivity for various pluripotency markers and alkaline phosphatase, as well as normal 44+ XY karyotype. Under the differentiation condition, the cells gave rise to cells of three germ layers. (c) 2017 The Authors. Published by Elsevier B. V. This is an open access article under the CC BY-NC-ND license.