Validation and Application of HPLC-ESI-MS/MS Method for the Determination of Irsogladine
- Authors
- Hoang, N. H.; Huong, N. L.; Hong, S. -Y.; Park, Je Won
- Issue Date
- 12월-2017
- Publisher
- AKADEMIAI KIADO RT
- Keywords
- Irsogladine; human plasma; HPLC-ESI-MS/MS; bioequivalence study
- Citation
- ACTA CHROMATOGRAPHICA, v.29, no.4, pp.459 - 462
- Indexed
- SCIE
SCOPUS
- Journal Title
- ACTA CHROMATOGRAPHICA
- Volume
- 29
- Number
- 4
- Start Page
- 459
- End Page
- 462
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/81459
- DOI
- 10.1556/1326.2017.00024
- ISSN
- 1233-2356
- Abstract
- A highly sensitive analytical tool for the fast quantification of irsogladine in human plasma was developed. Cleanup using a solid-phase extraction technique is a simple method for extracting both irsogladine and lamotrigine (internal standard) spiked into human plasma. The resolvable separation of both analytes through reversed-phase high-performance liquid chromatography (HPLC) was carried out within 5 min. The HPLC-electrospray ionization (ESI)-tandem mass spectrometry (MS/MS) method, which was operated in a selected reaction monitoring mode specific to the target analytes, was verified for use in the quantification of irsogladine. The inter-and intra-day precision (relative standard deviation, RSD) of irsogladine spiked into quality control samples were <7%, and their accuracies were between 96.6% and 102.1%. The calibration curve for irsogladine spiked into human plasma was linear over the range from 1.8 to 100 ng mL(-1) with lower limit of quantification at 1.8 ng mL(-1). The established method was successfully applied for a bioequivalence study of irsogladine.
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