Metabolic engineering of Enterobacter aerogenes for 2,3-butanediol production from sugarcane bagasse hydrolysate
DC Field | Value | Language |
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dc.contributor.author | Um, Jaeyong | - |
dc.contributor.author | Kim, Duck Gyun | - |
dc.contributor.author | Jung, Moo-Young | - |
dc.contributor.author | Saratale, Ganesh D. | - |
dc.contributor.author | Oh, Min-Kyu | - |
dc.date.accessioned | 2021-09-02T22:51:37Z | - |
dc.date.available | 2021-09-02T22:51:37Z | - |
dc.date.created | 2021-06-16 | - |
dc.date.issued | 2017-12 | - |
dc.identifier.issn | 0960-8524 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/81477 | - |
dc.description.abstract | The pathway engineering of Enterobacter aerogenes was attempted to improve its production capability of 2,3-butanediol from lignocellulosic biomass. In the medium containing glucose and xylose mixture as carbon sources, the gene deletion of pflB improved 2,3-butanediol carbon yield by 40%, while the deletion of ptsG increased xylose consumption rate significantly, improving the productivity at 12 hr by 70%. The constructed strain, EMY-22-galP, overexpressing glucose transporter (galP) in the triple gene knockout E. aerogenes, ldhA, pflB, and ptsG, provided the highest 2,3-butanediol titer and yield at 12 hr flask cultivation. Sugarcane bagasse was pretreated with green liquor, a solution containing Na2CO3 and Na2SO3 and was hydrolyzed by enzymes. The resulting hydrolysate was used as a carbon source for 2,3-butanediol production. After 72 hr in fermentation, the yield of 0.395 g/g sugar was achieved, suggesting an economic production of 2,3-butanediol was possible from lignocellulosic biomass with the metabolically engineered strain. (C) 2017 Elsevier Ltd. All rights reserved. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | ELSEVIER SCI LTD | - |
dc.subject | CARBON CATABOLITE REPRESSION | - |
dc.subject | HIGH-YIELD PRODUCTION | - |
dc.subject | MINERAL SALTS MEDIUM | - |
dc.subject | ESCHERICHIA-COLI | - |
dc.subject | SACCHAROMYCES-CEREVISIAE | - |
dc.subject | MICROBIAL-PRODUCTION | - |
dc.subject | EFFICIENT PRODUCTION | - |
dc.subject | KLEBSIELLA-OXYTOCA | - |
dc.subject | CORN STOVER | - |
dc.subject | FERMENTATION | - |
dc.title | Metabolic engineering of Enterobacter aerogenes for 2,3-butanediol production from sugarcane bagasse hydrolysate | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Oh, Min-Kyu | - |
dc.identifier.doi | 10.1016/j.biortech.2017.05.166 | - |
dc.identifier.scopusid | 2-s2.0-85020232848 | - |
dc.identifier.wosid | 000413038300034 | - |
dc.identifier.bibliographicCitation | BIORESOURCE TECHNOLOGY, v.245, pp.1567 - 1574 | - |
dc.relation.isPartOf | BIORESOURCE TECHNOLOGY | - |
dc.citation.title | BIORESOURCE TECHNOLOGY | - |
dc.citation.volume | 245 | - |
dc.citation.startPage | 1567 | - |
dc.citation.endPage | 1574 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Agriculture | - |
dc.relation.journalResearchArea | Biotechnology & Applied Microbiology | - |
dc.relation.journalResearchArea | Energy & Fuels | - |
dc.relation.journalWebOfScienceCategory | Agricultural Engineering | - |
dc.relation.journalWebOfScienceCategory | Biotechnology & Applied Microbiology | - |
dc.relation.journalWebOfScienceCategory | Energy & Fuels | - |
dc.subject.keywordPlus | CARBON CATABOLITE REPRESSION | - |
dc.subject.keywordPlus | HIGH-YIELD PRODUCTION | - |
dc.subject.keywordPlus | MINERAL SALTS MEDIUM | - |
dc.subject.keywordPlus | ESCHERICHIA-COLI | - |
dc.subject.keywordPlus | SACCHAROMYCES-CEREVISIAE | - |
dc.subject.keywordPlus | MICROBIAL-PRODUCTION | - |
dc.subject.keywordPlus | EFFICIENT PRODUCTION | - |
dc.subject.keywordPlus | KLEBSIELLA-OXYTOCA | - |
dc.subject.keywordPlus | CORN STOVER | - |
dc.subject.keywordPlus | FERMENTATION | - |
dc.subject.keywordAuthor | 2,3-Butanediol | - |
dc.subject.keywordAuthor | Enterobacter aerogenes | - |
dc.subject.keywordAuthor | Metabolic engineering | - |
dc.subject.keywordAuthor | Sugarcane bagasse | - |
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