Metabolic engineering of Enterobacter aerogenes for 2,3-butanediol production from sugarcane bagasse hydrolysate
- Authors
- Um, Jaeyong; Kim, Duck Gyun; Jung, Moo-Young; Saratale, Ganesh D.; Oh, Min-Kyu
- Issue Date
- 12월-2017
- Publisher
- ELSEVIER SCI LTD
- Keywords
- 2,3-Butanediol; Enterobacter aerogenes; Metabolic engineering; Sugarcane bagasse
- Citation
- BIORESOURCE TECHNOLOGY, v.245, pp.1567 - 1574
- Indexed
- SCIE
SCOPUS
- Journal Title
- BIORESOURCE TECHNOLOGY
- Volume
- 245
- Start Page
- 1567
- End Page
- 1574
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/81477
- DOI
- 10.1016/j.biortech.2017.05.166
- ISSN
- 0960-8524
- Abstract
- The pathway engineering of Enterobacter aerogenes was attempted to improve its production capability of 2,3-butanediol from lignocellulosic biomass. In the medium containing glucose and xylose mixture as carbon sources, the gene deletion of pflB improved 2,3-butanediol carbon yield by 40%, while the deletion of ptsG increased xylose consumption rate significantly, improving the productivity at 12 hr by 70%. The constructed strain, EMY-22-galP, overexpressing glucose transporter (galP) in the triple gene knockout E. aerogenes, ldhA, pflB, and ptsG, provided the highest 2,3-butanediol titer and yield at 12 hr flask cultivation. Sugarcane bagasse was pretreated with green liquor, a solution containing Na2CO3 and Na2SO3 and was hydrolyzed by enzymes. The resulting hydrolysate was used as a carbon source for 2,3-butanediol production. After 72 hr in fermentation, the yield of 0.395 g/g sugar was achieved, suggesting an economic production of 2,3-butanediol was possible from lignocellulosic biomass with the metabolically engineered strain. (C) 2017 Elsevier Ltd. All rights reserved.
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