Fluorescent Labeling of Protein Using Blue-Emitting 8-Amino-BODIPY Derivatives
- Authors
- Kim, Dokyoung; Ma, Donghee; Kim, Muwoong; Jung, Yuna; Kim, Na Hee; Lee, Chiho; Cho, Seo Won; Park, Sungnam; Huh, Youngbuhm; Jung, Junyang; Ahn, Kyo Han
- Issue Date
- 11월-2017
- Publisher
- SPRINGER/PLENUM PUBLISHERS
- Keywords
- labeling Protein labeling; Blue-emitting BODIPY; Fluorescent labeling; Bioconjugation
- Citation
- JOURNAL OF FLUORESCENCE, v.27, no.6, pp.2231 - 2238
- Indexed
- SCIE
SCOPUS
- Journal Title
- JOURNAL OF FLUORESCENCE
- Volume
- 27
- Number
- 6
- Start Page
- 2231
- End Page
- 2238
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/81657
- DOI
- 10.1007/s10895-017-2164-5
- ISSN
- 1053-0509
- Abstract
- 8-Amino-BODIPY (boron-dipyrromethane) dyes show bright blue fluorescence. Disclosed here are synthesis and characterization of the photophysical properties of a series of functionalized 8-Amino-BODIPY (BP1-4) for protein labeling. The compact structure and solvent-insensitive absorption property of the dye are desirable features for protein labeling. For the model protein, bovine serum albumin (BSA), the labeling proceeds under mild condition via amide bond formation or thiol-ene conjugation with maintaining the bright blue fluorescence. The chromatography and mass spectroscopy analysis clearly support the labeling of the BODIPY dye on the BSA. The protein labeling with blue-emitting BODIPY would be applicable for studying protein dynamics and fluorescence resonance energy transfer (FRET) with intrinsic biomolecules.
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