Physiological and Metabolomic Analysis of Issatchenkia orientalis MTY1 With Multiple Tolerance for Cellulosic Bioethanol Production
- Authors
- Seong, Yeong-Je; Lee, Hye-Jin; Lee, Jung-Eun; Kim, Sooah; Lee, Do Yup; Kim, Kyoung Heon; Park, Yong-Cheol
- Issue Date
- Nov-2017
- Publisher
- WILEY-V C H VERLAG GMBH
- Keywords
- Issatchenkia orientalis; multiple tolerance; fermentation; metabolomics; bioethanol
- Citation
- BIOTECHNOLOGY JOURNAL, v.12, no.11
- Indexed
- SCIE
SCOPUS
- Journal Title
- BIOTECHNOLOGY JOURNAL
- Volume
- 12
- Number
- 11
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/81791
- DOI
- 10.1002/biot.201700110
- ISSN
- 1860-6768
- Abstract
- Yeast with multiple tolerance onto harsh conditions has a number of advantages for bioethanol production. In this study, an alcohol yeast of Issatchenkia orientalis MTY1 is isolated in a Korean winery and its multiple tolerance against high temperature and acidic conditions is characterized in microaerobic batch cultures and by metabolomic analysis. In a series of batch cultures using 100 g L-1 glucose, I. orientalis MTY1 possesses wider growth ranges at pH 2-8 and 30-45 degrees C than a conventional yeast of Saccharomyces cerevisiae D452-2. Moreover, I. orientalis MTY1 showes higher cell growth and ethanol productivity in the presence of acetic acid or furfural than S. cerevisiae D452-2. I. orientalis MTY1 produces 41.4 g L-1 ethanol with 1.5 g L-1 h(-1) productivity at 42 degrees C and pH 4.2 in the presence of 4 g L-1 acetic acid, whereas a thermo-tolerant yeast of Kluyvermyces marxianus ATCC36907 does not grow. By metabolomics by GC-TOF MS and statistical analysis of 125 metabolite peaks, it is revealed that the thermo-tolerance of I. orientalis MTY1 might be ascribed to higher contents of unsaturated fatty acids, purines and pyrimidines than S. cerevisiae D452-2. Conclusively, I. orientalis MTY1 could be a potent workhorse with multiple tolerance against harsh conditions considered in cellulosic bioethanol production.
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