Preparation of phytosteryl ester and simultaneous enrichment of stearidonic acid via lipase-catalyzed esterification
DC Field | Value | Language |
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dc.contributor.author | Choi, Nakyung | - |
dc.contributor.author | Cho, Hyo Jung | - |
dc.contributor.author | Kim, Heejin | - |
dc.contributor.author | Kim, Yangha | - |
dc.contributor.author | Kim, Hak-Ryul | - |
dc.contributor.author | Kim, In-Hwan | - |
dc.date.accessioned | 2021-09-03T00:53:21Z | - |
dc.date.available | 2021-09-03T00:53:21Z | - |
dc.date.created | 2021-06-19 | - |
dc.date.issued | 2017-10 | - |
dc.identifier.issn | 1359-5113 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/82096 | - |
dc.description.abstract | The simultaneous synthesis of a phytosteryl ester and enrichment of stearidonic acid (SDA) were performed via a one-step lipase-catalyzed esterification of fatty acids from Ahiflower (TM) seed oil with phytosterol. A commercial lipase (Lipase OF) from Candida rugosa was employed as a biocatalyst. Three solvents were screened and cyclohexane was selected as a suitable reaction medium. The effects of enzyme loading, temperature, and solvent amount were investigated. The conversion as well as the SDA content were significantly improved by adding molecular sieves after 1 h of reaction. The optimum conditions were the enzyme loading of 10% (based on the total substrate weight), the temperature of 30 degrees C, and the solvent amount of 4 mL (based on a substrate weight of 2 g), respectively. The maximum conversion and SDA content in the residual fatty acid were 81 mol% and 58 mol%, respectively, under the optimum conditions. The SDA content increased a 3.4-fold from 17 mol% in Ahiflower' seed oil to 58 mol%. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | ELSEVIER SCI LTD | - |
dc.subject | POLYUNSATURATED FATTY-ACIDS | - |
dc.subject | CANDIDA-RUGOSA LIPASE | - |
dc.subject | ORGANIC-SOLVENTS | - |
dc.subject | FISH-OIL | - |
dc.subject | DHA | - |
dc.subject | CHOLESTEROL | - |
dc.subject | TRANSESTERIFICATION | - |
dc.subject | BIOCATALYSIS | - |
dc.subject | OPTIMIZATION | - |
dc.subject | ENZYMES | - |
dc.title | Preparation of phytosteryl ester and simultaneous enrichment of stearidonic acid via lipase-catalyzed esterification | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Kim, In-Hwan | - |
dc.identifier.doi | 10.1016/j.procbio.2017.06.013 | - |
dc.identifier.scopusid | 2-s2.0-85024111346 | - |
dc.identifier.wosid | 000413058400011 | - |
dc.identifier.bibliographicCitation | PROCESS BIOCHEMISTRY, v.61, pp.88 - 94 | - |
dc.relation.isPartOf | PROCESS BIOCHEMISTRY | - |
dc.citation.title | PROCESS BIOCHEMISTRY | - |
dc.citation.volume | 61 | - |
dc.citation.startPage | 88 | - |
dc.citation.endPage | 94 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Biotechnology & Applied Microbiology | - |
dc.relation.journalResearchArea | Engineering | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Biotechnology & Applied Microbiology | - |
dc.relation.journalWebOfScienceCategory | Engineering, Chemical | - |
dc.subject.keywordPlus | POLYUNSATURATED FATTY-ACIDS | - |
dc.subject.keywordPlus | CANDIDA-RUGOSA LIPASE | - |
dc.subject.keywordPlus | ORGANIC-SOLVENTS | - |
dc.subject.keywordPlus | FISH-OIL | - |
dc.subject.keywordPlus | DHA | - |
dc.subject.keywordPlus | CHOLESTEROL | - |
dc.subject.keywordPlus | TRANSESTERIFICATION | - |
dc.subject.keywordPlus | BIOCATALYSIS | - |
dc.subject.keywordPlus | OPTIMIZATION | - |
dc.subject.keywordPlus | ENZYMES | - |
dc.subject.keywordAuthor | Ahiflower (TM) seed oil | - |
dc.subject.keywordAuthor | Candida rugosa lipase | - |
dc.subject.keywordAuthor | Esterification | - |
dc.subject.keywordAuthor | Phytosterol | - |
dc.subject.keywordAuthor | Phytosteryl ester | - |
dc.subject.keywordAuthor | Stearidonic acid | - |
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