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Oxyresveratrol improves tight junction integrity through the PKC and MAPK signaling pathways in Caco-2 cells

Authors
Jo, HyunAHwang, DahyunKim, Jeong-KeunLim, Young-Hee
Issue Date
10월-2017
Publisher
PERGAMON-ELSEVIER SCIENCE LTD
Keywords
Oxyresveratrol; Tight junctions; Protein kinase C; Mitogen-activated protein kinase; Intestinal permeability
Citation
FOOD AND CHEMICAL TOXICOLOGY, v.108, pp.203 - 213
Indexed
SCIE
SCOPUS
Journal Title
FOOD AND CHEMICAL TOXICOLOGY
Volume
108
Start Page
203
End Page
213
URI
https://scholar.korea.ac.kr/handle/2021.sw.korea/82113
DOI
10.1016/j.fct.2017.08.002
ISSN
0278-6915
Abstract
Strengthening intestinal tight junctions (TJ) provides an effective barrier from the external environment and is important for recovery from inflammatory bowel disease. Oxyresveratrol (OXY), an isomer of hydroxylated resveratrol, is isolated from many plants. The aim of this study was to investigate the effect of OXY on intestinal TJ and to elucidate the mechanism underlying the OXY-mediated increase in TJ integrity in human intestinal Caco-2 cells. OXY-treated Caco-2 cell monolayers showed decreased monolayer permeability as evaluated by paracellular transport assay. The results showed that OXY significantly increased the levels of TJ-related genes and proteins (Claudin-1, Occludin and ZO-1) compared with those of the negative control. OXY activated protein kinase C (PKC) and increased expression levels of mitogen-activated protein kinase (MAPK) genes. OXY also increased gene and protein levels of the transcription factor Cdx-2. Expression levels of TJ, PKC and Cdx-2 proteins and trans epithelial electrical resistance (TEER) value decreased in OXY-treated Caco-2 cells following treatment with a pan-PKC inhibitor compared with those of the untreated control. In conclusion, OXY strengthens the integrity of the intestinal TJ barrier via activation of the PKC and MAPK pathways. (C) 2017 Elsevier Ltd. All rights reserved.
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LIM, YOUNG HEE
보건과학대학 (바이오시스템의과학부)
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