Two-Photon Probes for pH: Detection of Human Colon Cancer using Two-Photon Microscopy
DC Field | Value | Language |
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dc.contributor.author | Hong, Seung Taek | - |
dc.contributor.author | Kim, Tae Hyeong | - |
dc.contributor.author | Choi, Ji-Woo | - |
dc.contributor.author | Park, Seong Jun | - |
dc.contributor.author | Kwon, Sung An | - |
dc.contributor.author | Paik, Kyu Cheol | - |
dc.contributor.author | Han, Man So | - |
dc.contributor.author | Kim, Eun Sun | - |
dc.contributor.author | Chun, Hoon Jai | - |
dc.contributor.author | Heo, Jung-Nyoung | - |
dc.contributor.author | Cho, Bong Rae | - |
dc.date.accessioned | 2021-09-03T01:10:56Z | - |
dc.date.available | 2021-09-03T01:10:56Z | - |
dc.date.created | 2021-06-19 | - |
dc.date.issued | 2017-09-19 | - |
dc.identifier.issn | 0003-2700 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/82212 | - |
dc.description.abstract | We have developed two-photon (TP) pH-sensitive probes (BH-2 and BHEt-1) that exhibit absorption and emission maxima at 370 and 466 nm, and TP absorption cross-section values of 51 and 61 GM (1 GM = 10(-50)cm(4)s/photon), respectively, at 750 nm and pH 3.0 in a universal buffer (0.1 M citric acid, 0.1 M KH2PO4, 0.1 M Na2B4O7, 0.1 M Tris, 0.1 M KCl)/1,4-dioxane (7/3) solution. The TPM images of CCD-18co (a normal colon cell line) and HCT116 cells (a colon cancer cell line) labeled with BH-2 were too dim to be distinguished. When the same cells were labeled with BHEt-1, however, the TPM image of the HCT116 cells was Much brighter than that of CCD-18co cells, and the relative proportion of the acidic vesicles (P-acid) of the former was 5-fold larger than that of latter. BHEt-1 could also differentiate HepG2 cells (a human liver cancer cell line) from LX-2 cells (a human hepatic stellate cell line) with a 6-fold larger P-acid value. Human colon cancer tissues labeled with BHEt-1 showed similar results, demonstrating much brighter TPM images and 6-fold larger P-acid values compared to normal tissue. These results suggest the potential utility of BHEt-1 for detecting colon cancer in human tissues using TPM. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | AMER CHEMICAL SOC | - |
dc.subject | FLUORESCENT-PROBE | - |
dc.subject | LIVING CELLS | - |
dc.subject | LIVE CELLS | - |
dc.subject | ACIDIC PH | - |
dc.subject | IN-VIVO | - |
dc.subject | TISSUES | - |
dc.subject | MITOCHONDRIA | - |
dc.subject | LYSOSOMES | - |
dc.title | Two-Photon Probes for pH: Detection of Human Colon Cancer using Two-Photon Microscopy | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Kim, Eun Sun | - |
dc.contributor.affiliatedAuthor | Chun, Hoon Jai | - |
dc.contributor.affiliatedAuthor | Cho, Bong Rae | - |
dc.identifier.doi | 10.1021/acs.analchem.7b01804 | - |
dc.identifier.scopusid | 2-s2.0-85029573629 | - |
dc.identifier.wosid | 000411549100032 | - |
dc.identifier.bibliographicCitation | ANALYTICAL CHEMISTRY, v.89, no.18, pp.9830 - 9835 | - |
dc.relation.isPartOf | ANALYTICAL CHEMISTRY | - |
dc.citation.title | ANALYTICAL CHEMISTRY | - |
dc.citation.volume | 89 | - |
dc.citation.number | 18 | - |
dc.citation.startPage | 9830 | - |
dc.citation.endPage | 9835 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Analytical | - |
dc.subject.keywordPlus | FLUORESCENT-PROBE | - |
dc.subject.keywordPlus | LIVING CELLS | - |
dc.subject.keywordPlus | LIVE CELLS | - |
dc.subject.keywordPlus | ACIDIC PH | - |
dc.subject.keywordPlus | IN-VIVO | - |
dc.subject.keywordPlus | TISSUES | - |
dc.subject.keywordPlus | MITOCHONDRIA | - |
dc.subject.keywordPlus | LYSOSOMES | - |
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