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Two-Photon Probes for pH: Detection of Human Colon Cancer using Two-Photon Microscopy

Authors
Hong, Seung TaekKim, Tae HyeongChoi, Ji-WooPark, Seong JunKwon, Sung AnPaik, Kyu CheolHan, Man SoKim, Eun SunChun, Hoon JaiHeo, Jung-NyoungCho, Bong Rae
Issue Date
19-9월-2017
Publisher
AMER CHEMICAL SOC
Citation
ANALYTICAL CHEMISTRY, v.89, no.18, pp.9830 - 9835
Indexed
SCIE
SCOPUS
Journal Title
ANALYTICAL CHEMISTRY
Volume
89
Number
18
Start Page
9830
End Page
9835
URI
https://scholar.korea.ac.kr/handle/2021.sw.korea/82212
DOI
10.1021/acs.analchem.7b01804
ISSN
0003-2700
Abstract
We have developed two-photon (TP) pH-sensitive probes (BH-2 and BHEt-1) that exhibit absorption and emission maxima at 370 and 466 nm, and TP absorption cross-section values of 51 and 61 GM (1 GM = 10(-50)cm(4)s/photon), respectively, at 750 nm and pH 3.0 in a universal buffer (0.1 M citric acid, 0.1 M KH2PO4, 0.1 M Na2B4O7, 0.1 M Tris, 0.1 M KCl)/1,4-dioxane (7/3) solution. The TPM images of CCD-18co (a normal colon cell line) and HCT116 cells (a colon cancer cell line) labeled with BH-2 were too dim to be distinguished. When the same cells were labeled with BHEt-1, however, the TPM image of the HCT116 cells was Much brighter than that of CCD-18co cells, and the relative proportion of the acidic vesicles (P-acid) of the former was 5-fold larger than that of latter. BHEt-1 could also differentiate HepG2 cells (a human liver cancer cell line) from LX-2 cells (a human hepatic stellate cell line) with a 6-fold larger P-acid value. Human colon cancer tissues labeled with BHEt-1 showed similar results, demonstrating much brighter TPM images and 6-fold larger P-acid values compared to normal tissue. These results suggest the potential utility of BHEt-1 for detecting colon cancer in human tissues using TPM.
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