Study of Alzheimer's Disease-Related Biophysical Kinetics with a Microslit-Embedded Cantilever Sensor in a Liquid Environment
DC Field | Value | Language |
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dc.contributor.author | Chae, Myung-Sic | - |
dc.contributor.author | Kim, Jinsik | - |
dc.contributor.author | Yoo, Yong Kyoung | - |
dc.contributor.author | Lee, Jeong Hoon | - |
dc.contributor.author | Kim, Tae Geun | - |
dc.contributor.author | Hwang, Kyo Seon | - |
dc.date.accessioned | 2021-09-03T03:29:35Z | - |
dc.date.available | 2021-09-03T03:29:35Z | - |
dc.date.created | 2021-06-16 | - |
dc.date.issued | 2017-08 | - |
dc.identifier.issn | 1424-8220 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/82699 | - |
dc.description.abstract | A microsized slit-embedded cantilever sensor (slit cantilever) was fabricated and evaluated as a biosensing platform in a liquid environment. In order to minimize the degradation caused by viscous damping, a 300 x 100 mu m(2) (length x width) sized cantilever was released by a 5 mu m gap-surrounding and vibrated by an internal piezoelectric-driven self-actuator. Owing to the structure, when the single side of the slit cantilever was exposed to liquid a significant quality factor (Q = 35) could be achieved. To assess the sensing performance, the slit cantilever was exploited to study the biophysical kinetics related to A beta peptide. First, the quantification of A beta peptide with a concentration of 10 pg/mL to 1 mu g/mL was performed. The resonant responses exhibited a dynamic range from 100 pg/mL to 100 ng/mL (-56.5 to 774 Delta Hz) and a dissociation constant (K-D) of binding affinity was calculated as 1.75 nM. Finally, the A fi self-aggregation associated with AD pathogenesis was monitored by adding monomeric A fi peptides. As the concentration of added analyte increased from 100 ng/mL to 10 ng/mL, both the frequency shift values (813 to 1804 Delta Hz) and associate time constant increased. These results showed the excellent sensing performance of the slit cantilever overcoming a major drawback in liquid environments to become a promising diagnostic tool candidate. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | MDPI | - |
dc.subject | BETA | - |
dc.subject | AGGREGATION | - |
dc.subject | BIOSENSORS | - |
dc.subject | SYSTEMS | - |
dc.subject | SURFACE | - |
dc.subject | GROWTH | - |
dc.title | Study of Alzheimer's Disease-Related Biophysical Kinetics with a Microslit-Embedded Cantilever Sensor in a Liquid Environment | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Kim, Tae Geun | - |
dc.identifier.doi | 10.3390/s17081819 | - |
dc.identifier.scopusid | 2-s2.0-85027000993 | - |
dc.identifier.wosid | 000408576900122 | - |
dc.identifier.bibliographicCitation | SENSORS, v.17, no.8 | - |
dc.relation.isPartOf | SENSORS | - |
dc.citation.title | SENSORS | - |
dc.citation.volume | 17 | - |
dc.citation.number | 8 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.relation.journalResearchArea | Engineering | - |
dc.relation.journalResearchArea | Instruments & Instrumentation | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Analytical | - |
dc.relation.journalWebOfScienceCategory | Engineering, Electrical & Electronic | - |
dc.relation.journalWebOfScienceCategory | Instruments & Instrumentation | - |
dc.subject.keywordPlus | BETA | - |
dc.subject.keywordPlus | AGGREGATION | - |
dc.subject.keywordPlus | BIOSENSORS | - |
dc.subject.keywordPlus | SYSTEMS | - |
dc.subject.keywordPlus | SURFACE | - |
dc.subject.keywordPlus | GROWTH | - |
dc.subject.keywordAuthor | cantilever sensor | - |
dc.subject.keywordAuthor | biosensor | - |
dc.subject.keywordAuthor | viscous damping | - |
dc.subject.keywordAuthor | Alzheimer&apos | - |
dc.subject.keywordAuthor | s disease | - |
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