14-3-3 gamma regulates Copinel-mediated neuronal differentiation in HiB5 hippocampal progenitor cells
- Authors
- Yoo, Jae Cheal; Park, Nammi; Lee, Boah; Noshed, Abdullateef; Lee, Young-Sun; Kim, Tae Hwan; Lee, Da Yong; Kim, Ajung; Hwang, Eun Mi; Yi, Gwan-su; Park, Jae-Yong
- Issue Date
- 1-7월-2017
- Publisher
- ELSEVIER INC
- Keywords
- Copinel; 14-3-3 gamma; Protein-protein interaction; C2A domain; HiB5 cell
- Citation
- EXPERIMENTAL CELL RESEARCH, v.356, no.1, pp.85 - 92
- Indexed
- SCIE
SCOPUS
- Journal Title
- EXPERIMENTAL CELL RESEARCH
- Volume
- 356
- Number
- 1
- Start Page
- 85
- End Page
- 92
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/82863
- DOI
- 10.1016/j.yexcr.2017.04.015
- ISSN
- 0014-4827
- Abstract
- Copinel (CPNE1), known as a calcium-dependent membrane-binding protein, has tandem C2 domains and an A domain. We previously demonstrated that CPNE1 directly induces neuronal differentiation via Protein kinase B (AKT) phosphorylation in the hippocampal progenitor cell line, HiB5. To better understand its cellular function, we carried out a yeast two-hybrid screening to find CPNE1 binding partners. Among the identified proteins, 14-3-3 gamma appears to directly interact with CPNE1. Between CPNE1 and 14-3-3 gamma, the physical interaction as well as the specific binding regions of CPNE1 was confirmed in vitro and in vivo. Furthermore, among the seven 14-3-3 isotypes, only 14-3-3 gamma directly interacts with CPNE1. Our results also demonstrate that AKT phosphorylation, neurite outgrowth and expression of the neuronal marker protein are increased when 14-3-3 gamma is overexpressed in CPNE1 high expressed HiB5 cells. Furthermore, the neighboring Ser54 amino acids residue of C2A domain in CPNE1 has an important role in binding with 14-3-3 gamma, and in differentiation-related function of CPNE1. Moreover, mutation of Ser54 amino acids residue in CPNE1 effectively decreased association with 14-3-3 gamma and neuronal differentiation of HiB5 cells. Collectively, our findings indicate that 14-3-3 gamma regulates the differentiation ability of CPNE1 through the binding with C2A domain of CPNE1 in HiB5 cells.
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Collections - College of Health Sciences > School of Biosystems and Biomedical Sciences > 1. Journal Articles
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