Nocardioides litoris sp nov., isolated from the Taean seashore

Abstract

A novel Gram-stain-positive, irregularly rod- shaped actinomycete, designated strain 002-2(T), was isolated from sand beach sediment collected from the Taean seashore, Republic of Korea. Cells were aerobic, oxidase-negative and catalase- positive. Colonies of cells were bright yellow, circular, smooth and convex. The diagnostic diamino acid in the cell wall was LLdiaminopimelic acid. The predominant menaquinone was MK-8( H-4). The polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, an unknown phospholipid and two unknown amino phospholipids. The major fatty acids were iso-C-16:0 and C-17:1 omega 8c. The DNA G+C content was 68.8 mol%. 16S rRNA gene sequence analysis revealed that strain 002- 2(T) belongs to the family Nocardioidaceae and formed a cluster with Nocardioides rubroscoriae ScoA25(T) (98.1% sequence similarity) and Nocardioides plantarum NCIMB 12834(T) (97.6 %). On the basis of the phenotypic and phylogenetic data, strain 002-2(T) (= KCTC 39838(T) = DSM 103718(T)) is considered to represent a novel species of the genus Nocardioides, for which the name Nocardioides litoris sp. nov. is proposed.