Thalassotalea crassostreae sp nov., isolated from Pacific oyster

Abstract

A Gram-staining-negative, aerobic, rod-shaped bacterial strain, designated LPB0090(T), was isolated from the Pacific oyster, Crassostreagigas, collected from the Yeongheung Island, Korea (37 degrees 15' 16.1 '' N; 126 degrees 29' 46.5 '' E). The complete genome sequence of LPB0090(T) (accession number CP017689) was 3 861 670 bp long with a DNA G+C content of 38.8 mol%. The genome included 3245 protein-coding genes and six copies of rRNA operons. On the basis of the results of 16S rRNA gene sequence analysis, LPB0090(T) was found to form an independent phyletic line within the genus Thalassotalea, with 94.7-96.0% sequence similarities to the previously known species of the genus. The isoprenoid quinone (Q-8) and major fatty acids (C-16 : 0, C-17 : 1 omega 8c, and C-16 : 1 omega 7c and/or C-16 : 1 omega 6c) of the isolate were similar to those of the other members of the genus Thalassotalea. A number of phenotypic features, however, distinguished LPB0090(T) from its closest neighbour Thalassotalea ponticola as well as other species of the genus Thalassotalea. On the basis of the phylogenetic, genomic and phenotypic data presented in this study, the strain was classified as representing a novel species of the genus Thalassotalea. Therefore, the name Thalassotalea crassostreae sp. nov. is proposed for the isolate. The type strain is LPB0090(T) (=KACC 18695(T)=JCM 31189(T)).