Molecular cloning and characterization of a novel cold-active lipase from Pichia lynferdii NRRL Y-7723
DC Field | Value | Language |
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dc.contributor.author | Bae, Jae-Han | - |
dc.contributor.author | Kim, In-Hwan | - |
dc.contributor.author | Lee, Ki-Teak | - |
dc.contributor.author | Hou, Ching T. | - |
dc.contributor.author | Kim, Hak-Ryul | - |
dc.date.accessioned | 2021-09-03T04:27:54Z | - |
dc.date.available | 2021-09-03T04:27:54Z | - |
dc.date.created | 2021-06-16 | - |
dc.date.issued | 2017-07 | - |
dc.identifier.issn | 1878-8181 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/82958 | - |
dc.description.abstract | Lipase is one of the widely used biocatalysts, which is well studied for its application in industrial production. Recently, lipases with special characteristics such as thermo-stability, alkaline-, acidic nature, and cold-activity, have gained attention for effective applications in specific purposes. Previously, Pichia lynferdii NRRL Y-7723 was reported to produce high amounts of extracellular cold-active lipase (Kim et al., 2010). In this study, we report the identification of lip1 gene that encodes an extracellular cold-active lipase from P. lynferdii NRRL Y-7723. The open reading frame of the gene consisted of 1122 bp of nucleotides that encoded a protein with 373 amino acids. The deduced molecular weight and isoelectric point were 41.8 kDa and pH 5.82, respectively. The lip1 gene was cloned into a bacterial expression vector, and the recombinant lipase was successfully expressed and purified. Several parameters of the recombinant lipase were analyzed, and Lip1 showed high activity at low temperature. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | ELSEVIER SCIENCE BV | - |
dc.subject | ESCHERICHIA-COLI | - |
dc.subject | ADAPTED LIPASE | - |
dc.subject | SACCHAROMYCES-CEREVISIAE | - |
dc.subject | ANTARCTIC BACTERIA | - |
dc.subject | MORAXELLA TA144 | - |
dc.subject | GENE CLONING | - |
dc.subject | EXPRESSION | - |
dc.subject | PURIFICATION | - |
dc.subject | SEQUENCE | - |
dc.subject | ENZYMES | - |
dc.title | Molecular cloning and characterization of a novel cold-active lipase from Pichia lynferdii NRRL Y-7723 | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Kim, In-Hwan | - |
dc.identifier.doi | 10.1016/j.bcab.2017.05.008 | - |
dc.identifier.scopusid | 2-s2.0-85020053628 | - |
dc.identifier.wosid | 000414500200003 | - |
dc.identifier.bibliographicCitation | BIOCATALYSIS AND AGRICULTURAL BIOTECHNOLOGY, v.11, pp.19 - 25 | - |
dc.relation.isPartOf | BIOCATALYSIS AND AGRICULTURAL BIOTECHNOLOGY | - |
dc.citation.title | BIOCATALYSIS AND AGRICULTURAL BIOTECHNOLOGY | - |
dc.citation.volume | 11 | - |
dc.citation.startPage | 19 | - |
dc.citation.endPage | 25 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biotechnology & Applied Microbiology | - |
dc.relation.journalWebOfScienceCategory | Biotechnology & Applied Microbiology | - |
dc.subject.keywordPlus | ESCHERICHIA-COLI | - |
dc.subject.keywordPlus | ADAPTED LIPASE | - |
dc.subject.keywordPlus | SACCHAROMYCES-CEREVISIAE | - |
dc.subject.keywordPlus | ANTARCTIC BACTERIA | - |
dc.subject.keywordPlus | MORAXELLA TA144 | - |
dc.subject.keywordPlus | GENE CLONING | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | PURIFICATION | - |
dc.subject.keywordPlus | SEQUENCE | - |
dc.subject.keywordPlus | ENZYMES | - |
dc.subject.keywordAuthor | Lipase | - |
dc.subject.keywordAuthor | Cold-active | - |
dc.subject.keywordAuthor | Cloning | - |
dc.subject.keywordAuthor | Pichia lynferdii | - |
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