Biphasic Calcium Phosphate (BCP)-Immobilized Porous Poly (D,L-Lactic-co-Glycolic Acid) Microspheres Enhance Osteogenic Activities of Osteoblasts

Abstract

The purpose of this study was to evaluate the potential of porous poly (D, L-lactic-co-glycolic acid) (PLGA) microspheres (PMSs) immobilized on biphasic calcium phosphate nanoparticles (BCP NPs) (BCP-IM-PMSs) to enhance osteogenic activity. PMSs were fabricated using a fluidic device, and their surfaces were modified with L-lysine (aminated-PMSs), whereas the BCP NPs were modified with heparin-dopamine (Hep-DOPA) to obtain heparinized-BCP (Hep-BCP) NPs. BCP-IM-PMSs were fabricated via electrostatic interactions between the Hep-BCP NPs and aminated-PMSs. The fabricated BCP-IM-PMSs showed an interconnected pore structure. In vitro studies showed that MG-63 cells cultured on BCP-IM-PMSs had increased alkaline phosphatase activity, calcium content, and mRNA expression of osteocalcin (OCN) and osteopontin (OPN) compared with cells cultured on PMSs. These data suggest that BCP NP-immobilized PMSs have the potential to enhance osteogenic activity.