Biphasic Calcium Phosphate (BCP)-Immobilized Porous Poly (D,L-Lactic-co-Glycolic Acid) Microspheres Enhance Osteogenic Activities of Osteoblasts
- Authors
- Shim, Kyu-Sik; Kim, Sung Eun; Yun, Young-Pil; Choi, Somang; Kim, Hak-Jun; Park, Kyeongsoon; Song, Hae-Ryong
- Issue Date
- 7월-2017
- Publisher
- MDPI AG
- Keywords
- porous microspheres (PMSs); biphasic calcium phosphate (BCP); MG-63 cells; osteogenic activity
- Citation
- POLYMERS, v.9, no.7
- Indexed
- SCIE
SCOPUS
- Journal Title
- POLYMERS
- Volume
- 9
- Number
- 7
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/83034
- DOI
- 10.3390/polym9070297
- ISSN
- 2073-4360
- Abstract
- The purpose of this study was to evaluate the potential of porous poly (D, L-lactic-co-glycolic acid) (PLGA) microspheres (PMSs) immobilized on biphasic calcium phosphate nanoparticles (BCP NPs) (BCP-IM-PMSs) to enhance osteogenic activity. PMSs were fabricated using a fluidic device, and their surfaces were modified with L-lysine (aminated-PMSs), whereas the BCP NPs were modified with heparin-dopamine (Hep-DOPA) to obtain heparinized-BCP (Hep-BCP) NPs. BCP-IM-PMSs were fabricated via electrostatic interactions between the Hep-BCP NPs and aminated-PMSs. The fabricated BCP-IM-PMSs showed an interconnected pore structure. In vitro studies showed that MG-63 cells cultured on BCP-IM-PMSs had increased alkaline phosphatase activity, calcium content, and mRNA expression of osteocalcin (OCN) and osteopontin (OPN) compared with cells cultured on PMSs. These data suggest that BCP NP-immobilized PMSs have the potential to enhance osteogenic activity.
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Collections - College of Medicine > Department of Medical Science > 1. Journal Articles
- Graduate School > Department of Biomedical Sciences > 1. Journal Articles
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