Genetic Assembly of Double-Layered Fluorescent Protein Nanoparticles for Cancer Targeting and Imaging
DC Field | Value | Language |
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dc.contributor.author | Kim, Seong-Eun | - |
dc.contributor.author | Jo, Sung Duk | - |
dc.contributor.author | Kwon, Koo Chul | - |
dc.contributor.author | Won, You-Yeon | - |
dc.contributor.author | Lee, Jeewon | - |
dc.date.accessioned | 2021-09-03T06:45:08Z | - |
dc.date.available | 2021-09-03T06:45:08Z | - |
dc.date.created | 2021-06-16 | - |
dc.date.issued | 2017-05 | - |
dc.identifier.issn | 2198-3844 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/83611 | - |
dc.description.abstract | Hepatitis B virus capsid (HBVC), a self-assembled protein nanoparticle comprised of 180 or 240 subunit proteins, is used as a cage for genetic encapsulation of fluorescent proteins (FPs). The self-quenching of FPs is controlled by varying the spacing between FPs within the capsid structure. Double-layered FP nanoparticle possessing cancer cell-targeting capabilities is also produced by additionally attaching FPs and cancer cell receptor-binding peptides (affibodies) to the outer surface of the capsid. The generically modified HBVC with double layers of mCardinal FPs and affibodies (mC-DL-HBVC) exhibit a high fluorescence intensity and a strong photostability, and is efficiently internalized by cancer cells and significantly stable against intracellular degradation. The mC-DL-HBVC effectively detects tumor in live mice with enhanced tumor targeting and imaging efficiency with far less accumulation in the liver, compared to a conventional fluorescent dye, Cy5.5. This suggests the great potential of mC-DL-HBVC as a promising contrast agent for in vivo tumor fluorescence imaging. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | WILEY | - |
dc.subject | VIRAL NANOPARTICLES | - |
dc.subject | GUIDED SURGERY | - |
dc.subject | PARTICLES | - |
dc.title | Genetic Assembly of Double-Layered Fluorescent Protein Nanoparticles for Cancer Targeting and Imaging | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Lee, Jeewon | - |
dc.identifier.doi | 10.1002/advs.201600471 | - |
dc.identifier.scopusid | 2-s2.0-85013272393 | - |
dc.identifier.wosid | 000402740300009 | - |
dc.identifier.bibliographicCitation | ADVANCED SCIENCE, v.4, no.5 | - |
dc.relation.isPartOf | ADVANCED SCIENCE | - |
dc.citation.title | ADVANCED SCIENCE | - |
dc.citation.volume | 4 | - |
dc.citation.number | 5 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.relation.journalResearchArea | Science & Technology - Other Topics | - |
dc.relation.journalResearchArea | Materials Science | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Multidisciplinary | - |
dc.relation.journalWebOfScienceCategory | Nanoscience & Nanotechnology | - |
dc.relation.journalWebOfScienceCategory | Materials Science, Multidisciplinary | - |
dc.subject.keywordPlus | VIRAL NANOPARTICLES | - |
dc.subject.keywordPlus | GUIDED SURGERY | - |
dc.subject.keywordPlus | PARTICLES | - |
dc.subject.keywordAuthor | cancer targeting and imaging | - |
dc.subject.keywordAuthor | double-layered fluorescent proteins | - |
dc.subject.keywordAuthor | genetic encapsulation | - |
dc.subject.keywordAuthor | super-fluorescent protein nanoparticles | - |
dc.subject.keywordAuthor | viral capsid | - |
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