Genetic Assembly of Double-Layered Fluorescent Protein Nanoparticles for Cancer Targeting and Imaging
- Authors
- Kim, Seong-Eun; Jo, Sung Duk; Kwon, Koo Chul; Won, You-Yeon; Lee, Jeewon
- Issue Date
- 5월-2017
- Publisher
- WILEY
- Keywords
- cancer targeting and imaging; double-layered fluorescent proteins; genetic encapsulation; super-fluorescent protein nanoparticles; viral capsid
- Citation
- ADVANCED SCIENCE, v.4, no.5
- Indexed
- SCIE
SCOPUS
- Journal Title
- ADVANCED SCIENCE
- Volume
- 4
- Number
- 5
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/83611
- DOI
- 10.1002/advs.201600471
- ISSN
- 2198-3844
- Abstract
- Hepatitis B virus capsid (HBVC), a self-assembled protein nanoparticle comprised of 180 or 240 subunit proteins, is used as a cage for genetic encapsulation of fluorescent proteins (FPs). The self-quenching of FPs is controlled by varying the spacing between FPs within the capsid structure. Double-layered FP nanoparticle possessing cancer cell-targeting capabilities is also produced by additionally attaching FPs and cancer cell receptor-binding peptides (affibodies) to the outer surface of the capsid. The generically modified HBVC with double layers of mCardinal FPs and affibodies (mC-DL-HBVC) exhibit a high fluorescence intensity and a strong photostability, and is efficiently internalized by cancer cells and significantly stable against intracellular degradation. The mC-DL-HBVC effectively detects tumor in live mice with enhanced tumor targeting and imaging efficiency with far less accumulation in the liver, compared to a conventional fluorescent dye, Cy5.5. This suggests the great potential of mC-DL-HBVC as a promising contrast agent for in vivo tumor fluorescence imaging.
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Collections - College of Engineering > Department of Chemical and Biological Engineering > 1. Journal Articles
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