Crystal structure of the EnvZ periplasmic domain with CHAPS
DC Field | Value | Language |
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dc.contributor.author | Hwang, Eunha | - |
dc.contributor.author | Cheong, Hae-Kap | - |
dc.contributor.author | Kim, Sang-Yoon | - |
dc.contributor.author | Kwon, Ohsuk | - |
dc.contributor.author | Blain, Katherine Y. | - |
dc.contributor.author | Choe, Senyon | - |
dc.contributor.author | Yeo, Kwon Joo | - |
dc.contributor.author | Jung, Yong Woo | - |
dc.contributor.author | Jeon, Young Ho | - |
dc.contributor.author | Cheong, Chaejoon | - |
dc.date.accessioned | 2021-09-03T06:45:18Z | - |
dc.date.available | 2021-09-03T06:45:18Z | - |
dc.date.created | 2021-06-16 | - |
dc.date.issued | 2017-05 | - |
dc.identifier.issn | 0014-5793 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/83612 | - |
dc.description.abstract | Bacteria sense and respond to osmolarity through the EnvZ-OmpR two-component system. The structure of the periplasmic sensor domain of EnvZ (EnvZ-PD) is not available yet. Here, we present the crystal structure of EnvZ-PD in the presence of CHAPS detergent. The structure of EnvZ-PD shows similar folding topology to the PDC domains of PhoQ, DcuS, and CitA, but distinct orientations of helices and beta-hairpin structures. The CD and NMR spectra of EnvZ-PD in the presence of cholate, a major component of bile salts, are similar to those with CHAPS. Chemical cross-linking shows that the dimerization of EnvZ-PD is significantly inhibited by the CHAPS and cholate. Together with beta-galactosidase assay, these results suggest that bile salts may affect the EnvZ structure and function in Escherichia coli. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | WILEY | - |
dc.subject | TRANSMEMBRANE SIGNAL-TRANSDUCTION | - |
dc.subject | HISTIDINE KINASE ENVZ | - |
dc.subject | PORIN GENE-EXPRESSION | - |
dc.subject | ESCHERICHIA-COLI | - |
dc.subject | SENSOR | - |
dc.subject | OMPR | - |
dc.subject | BACTERIAL | - |
dc.subject | FLUORESCENCE | - |
dc.subject | TRANSITIONS | - |
dc.subject | PROTEIN | - |
dc.title | Crystal structure of the EnvZ periplasmic domain with CHAPS | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Yeo, Kwon Joo | - |
dc.contributor.affiliatedAuthor | Jung, Yong Woo | - |
dc.contributor.affiliatedAuthor | Jeon, Young Ho | - |
dc.identifier.doi | 10.1002/1873-3468.12658 | - |
dc.identifier.scopusid | 2-s2.0-85019105528 | - |
dc.identifier.wosid | 000403353700009 | - |
dc.identifier.bibliographicCitation | FEBS LETTERS, v.591, no.10, pp.1419 - 1428 | - |
dc.relation.isPartOf | FEBS LETTERS | - |
dc.citation.title | FEBS LETTERS | - |
dc.citation.volume | 591 | - |
dc.citation.number | 10 | - |
dc.citation.startPage | 1419 | - |
dc.citation.endPage | 1428 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Biophysics | - |
dc.relation.journalResearchArea | Cell Biology | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Biophysics | - |
dc.relation.journalWebOfScienceCategory | Cell Biology | - |
dc.subject.keywordPlus | TRANSMEMBRANE SIGNAL-TRANSDUCTION | - |
dc.subject.keywordPlus | HISTIDINE KINASE ENVZ | - |
dc.subject.keywordPlus | PORIN GENE-EXPRESSION | - |
dc.subject.keywordPlus | ESCHERICHIA-COLI | - |
dc.subject.keywordPlus | SENSOR | - |
dc.subject.keywordPlus | OMPR | - |
dc.subject.keywordPlus | BACTERIAL | - |
dc.subject.keywordPlus | FLUORESCENCE | - |
dc.subject.keywordPlus | TRANSITIONS | - |
dc.subject.keywordPlus | PROTEIN | - |
dc.subject.keywordAuthor | EnvZ | - |
dc.subject.keywordAuthor | periplasmic domain | - |
dc.subject.keywordAuthor | X-ray crystallography | - |
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