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Crystal structure of the EnvZ periplasmic domain with CHAPS

Authors
Hwang, EunhaCheong, Hae-KapKim, Sang-YoonKwon, OhsukBlain, Katherine Y.Choe, SenyonYeo, Kwon JooJung, Yong WooJeon, Young HoCheong, Chaejoon
Issue Date
5월-2017
Publisher
WILEY
Keywords
EnvZ; periplasmic domain; X-ray crystallography
Citation
FEBS LETTERS, v.591, no.10, pp.1419 - 1428
Indexed
SCIE
SCOPUS
Journal Title
FEBS LETTERS
Volume
591
Number
10
Start Page
1419
End Page
1428
URI
https://scholar.korea.ac.kr/handle/2021.sw.korea/83612
DOI
10.1002/1873-3468.12658
ISSN
0014-5793
Abstract
Bacteria sense and respond to osmolarity through the EnvZ-OmpR two-component system. The structure of the periplasmic sensor domain of EnvZ (EnvZ-PD) is not available yet. Here, we present the crystal structure of EnvZ-PD in the presence of CHAPS detergent. The structure of EnvZ-PD shows similar folding topology to the PDC domains of PhoQ, DcuS, and CitA, but distinct orientations of helices and beta-hairpin structures. The CD and NMR spectra of EnvZ-PD in the presence of cholate, a major component of bile salts, are similar to those with CHAPS. Chemical cross-linking shows that the dimerization of EnvZ-PD is significantly inhibited by the CHAPS and cholate. Together with beta-galactosidase assay, these results suggest that bile salts may affect the EnvZ structure and function in Escherichia coli.
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