Transcriptional analysis of genes encoding beta-glucosidase of Schizophyllum commune KUC9397 under optimal conditions
DC Field | Value | Language |
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dc.contributor.author | Lee, Young Min | - |
dc.contributor.author | Lee, Hanbyul | - |
dc.contributor.author | Heo, Young Mok | - |
dc.contributor.author | Lee, Hwanhwi | - |
dc.contributor.author | Hong, Joo-Hyun | - |
dc.contributor.author | Kim, Jae-Jin | - |
dc.date.accessioned | 2021-09-03T07:01:27Z | - |
dc.date.available | 2021-09-03T07:01:27Z | - |
dc.date.created | 2021-06-16 | - |
dc.date.issued | 2017-05 | - |
dc.identifier.issn | 0015-5632 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/83704 | - |
dc.description.abstract | The present study was conducted to determine the gene responsible for beta-glucosidase (BGL) production and to generate a full-length complementary DNA (cDNA) of one of the putative BGL genes, which showed a significant expression level when Schizophyllum commune KUC9397 was grown in optimized medium. The relative expression levels of seven genes encoding BGL of S. commune KUC9397 were determined with real-time quantitative reverse transcription PCR in cellulose-containing optimized medium (OM) compared to glucose-containing basal medium (BM). The most abundant transcript was bgl3a in OM. The transcript number of the bgl3a increased more than 57.60-fold when S. commune KUC9397 was grown on cellulose-containing OM compared to that on glucose-containing BM. The bgl3a was identified, and a deduced amino acid sequence of bgl3a shared homology (97%) with GH3 BGL of S. commune H4-8. This is the first report showing the transcription levels of genes encoding BGL and identification of full-length cDNA of glycoside hydrolase 3 (GH3) BGL from S. commune. Furthermore, this study is one of the steps for consolidated bioprocessing of lignocellulosic biomass to bioethanol. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | SPRINGER | - |
dc.subject | CELLOBIOSE DEHYDROGENASE | - |
dc.subject | TRICHODERMA-REESEI | - |
dc.subject | EXPRESSION | - |
dc.subject | CLONING | - |
dc.subject | OPTIMIZATION | - |
dc.subject | DEGRADATION | - |
dc.subject | CELLULASE | - |
dc.subject | INDUCTION | - |
dc.subject | SEQUENCE | - |
dc.subject | ENZYMES | - |
dc.title | Transcriptional analysis of genes encoding beta-glucosidase of Schizophyllum commune KUC9397 under optimal conditions | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Lee, Young Min | - |
dc.contributor.affiliatedAuthor | Kim, Jae-Jin | - |
dc.identifier.doi | 10.1007/s12223-016-0484-5 | - |
dc.identifier.scopusid | 2-s2.0-85000869853 | - |
dc.identifier.wosid | 000399241200003 | - |
dc.identifier.bibliographicCitation | FOLIA MICROBIOLOGICA, v.62, no.3, pp.191 - 196 | - |
dc.relation.isPartOf | FOLIA MICROBIOLOGICA | - |
dc.citation.title | FOLIA MICROBIOLOGICA | - |
dc.citation.volume | 62 | - |
dc.citation.number | 3 | - |
dc.citation.startPage | 191 | - |
dc.citation.endPage | 196 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biotechnology & Applied Microbiology | - |
dc.relation.journalResearchArea | Microbiology | - |
dc.relation.journalWebOfScienceCategory | Biotechnology & Applied Microbiology | - |
dc.relation.journalWebOfScienceCategory | Microbiology | - |
dc.subject.keywordPlus | CELLOBIOSE DEHYDROGENASE | - |
dc.subject.keywordPlus | TRICHODERMA-REESEI | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | CLONING | - |
dc.subject.keywordPlus | OPTIMIZATION | - |
dc.subject.keywordPlus | DEGRADATION | - |
dc.subject.keywordPlus | CELLULASE | - |
dc.subject.keywordPlus | INDUCTION | - |
dc.subject.keywordPlus | SEQUENCE | - |
dc.subject.keywordPlus | ENZYMES | - |
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