Transcriptional analysis of genes encoding beta-glucosidase of Schizophyllum commune KUC9397 under optimal conditions
- Authors
- Lee, Young Min; Lee, Hanbyul; Heo, Young Mok; Lee, Hwanhwi; Hong, Joo-Hyun; Kim, Jae-Jin
- Issue Date
- 5월-2017
- Publisher
- SPRINGER
- Citation
- FOLIA MICROBIOLOGICA, v.62, no.3, pp.191 - 196
- Indexed
- SCIE
SCOPUS
- Journal Title
- FOLIA MICROBIOLOGICA
- Volume
- 62
- Number
- 3
- Start Page
- 191
- End Page
- 196
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/83704
- DOI
- 10.1007/s12223-016-0484-5
- ISSN
- 0015-5632
- Abstract
- The present study was conducted to determine the gene responsible for beta-glucosidase (BGL) production and to generate a full-length complementary DNA (cDNA) of one of the putative BGL genes, which showed a significant expression level when Schizophyllum commune KUC9397 was grown in optimized medium. The relative expression levels of seven genes encoding BGL of S. commune KUC9397 were determined with real-time quantitative reverse transcription PCR in cellulose-containing optimized medium (OM) compared to glucose-containing basal medium (BM). The most abundant transcript was bgl3a in OM. The transcript number of the bgl3a increased more than 57.60-fold when S. commune KUC9397 was grown on cellulose-containing OM compared to that on glucose-containing BM. The bgl3a was identified, and a deduced amino acid sequence of bgl3a shared homology (97%) with GH3 BGL of S. commune H4-8. This is the first report showing the transcription levels of genes encoding BGL and identification of full-length cDNA of glycoside hydrolase 3 (GH3) BGL from S. commune. Furthermore, this study is one of the steps for consolidated bioprocessing of lignocellulosic biomass to bioethanol.
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Collections - College of Life Sciences and Biotechnology > Division of Environmental Science and Ecological Engineering > 1. Journal Articles
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