Single wall carbon nanotube electrode system capable of quantitative detection of CD4(+) T cells
DC Field | Value | Language |
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dc.contributor.author | Kim, Joonhyub | - |
dc.contributor.author | Park, Gayoung | - |
dc.contributor.author | Lee, Seoho | - |
dc.contributor.author | Hwang, Suk-Won | - |
dc.contributor.author | Min, Namki | - |
dc.contributor.author | Lee, Kyung-Mi | - |
dc.date.accessioned | 2021-09-03T07:09:29Z | - |
dc.date.available | 2021-09-03T07:09:29Z | - |
dc.date.created | 2021-06-16 | - |
dc.date.issued | 2017-04-15 | - |
dc.identifier.issn | 0956-5663 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/83748 | - |
dc.description.abstract | Development of CNT-based CD4(+) T cell imunosensors remains in its infancy due to the poor immobilization efficiency, lack of reproducibility, and difficulty in providing linear quantification. Here, we developed a fully integrated single wall carbon nanotube (SWCNT)-based immunosensor capable of selective capture and linear quantification of CD4(+)T cells with greater dynamic range. By employing repeated two-step oxygen (O-2) plasma treatment processes with 35 days of recovery periods, we achieved the enhanced functionalization of the CNT surface and the removal of the byproduct of spray-coated SWCNTs that hinders charge transfer and stable CD4(+) T cell sensing. As a result, a linear electrochemical signal was generated in direct proportion to the bound cells. The slope of a SWCNT electrode in a target concentration range (10(2)similar to 10(6) cells/mL) was 4.55 x 10(-2) A per concentration decade, with the lowest detection limit of 1 x 10(2) cells/mL. Since the reduced number of CD4(+) T cell counts in patients' peripheral blood corresponds to the progression of HIV disease, our CD4(+) T cell-immunosensor provides a simple and low-cost platform which can fulfill the requirement for the development of point-of-care (POC) diagnostic technologies for human immunodeficiency virus (HIV) patients in resource limited countries. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | ELSEVIER ADVANCED TECHNOLOGY | - |
dc.subject | INFECTED INDIVIDUALS | - |
dc.subject | BIOSENSORS | - |
dc.subject | DIAGNOSIS | - |
dc.subject | IMPEDANCE | - |
dc.subject | SENSORS | - |
dc.subject | ENZYME | - |
dc.subject | ACID | - |
dc.title | Single wall carbon nanotube electrode system capable of quantitative detection of CD4(+) T cells | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Hwang, Suk-Won | - |
dc.contributor.affiliatedAuthor | Min, Namki | - |
dc.contributor.affiliatedAuthor | Lee, Kyung-Mi | - |
dc.identifier.doi | 10.1016/j.bios.2016.11.055 | - |
dc.identifier.scopusid | 2-s2.0-84999810238 | - |
dc.identifier.wosid | 000392768000032 | - |
dc.identifier.bibliographicCitation | BIOSENSORS & BIOELECTRONICS, v.90, pp.238 - 244 | - |
dc.relation.isPartOf | BIOSENSORS & BIOELECTRONICS | - |
dc.citation.title | BIOSENSORS & BIOELECTRONICS | - |
dc.citation.volume | 90 | - |
dc.citation.startPage | 238 | - |
dc.citation.endPage | 244 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biophysics | - |
dc.relation.journalResearchArea | Biotechnology & Applied Microbiology | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.relation.journalResearchArea | Electrochemistry | - |
dc.relation.journalResearchArea | Science & Technology - Other Topics | - |
dc.relation.journalWebOfScienceCategory | Biophysics | - |
dc.relation.journalWebOfScienceCategory | Biotechnology & Applied Microbiology | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Analytical | - |
dc.relation.journalWebOfScienceCategory | Electrochemistry | - |
dc.relation.journalWebOfScienceCategory | Nanoscience & Nanotechnology | - |
dc.subject.keywordPlus | INFECTED INDIVIDUALS | - |
dc.subject.keywordPlus | BIOSENSORS | - |
dc.subject.keywordPlus | DIAGNOSIS | - |
dc.subject.keywordPlus | IMPEDANCE | - |
dc.subject.keywordPlus | SENSORS | - |
dc.subject.keywordPlus | ENZYME | - |
dc.subject.keywordPlus | ACID | - |
dc.subject.keywordAuthor | CD4(+) T cells | - |
dc.subject.keywordAuthor | SWCNT | - |
dc.subject.keywordAuthor | Electrochemical analysis | - |
dc.subject.keywordAuthor | O-2 plasma treatment | - |
dc.subject.keywordAuthor | T cell immunosensor | - |
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