배양된 신경세포 관찰을 위한 초고압전자현미경 홀마운트 시료제작기법Whole Mount Preparation of Primary Cultured Neuron for HVEM Observation
- Other Titles
- Whole Mount Preparation of Primary Cultured Neuron for HVEM Observation
- Authors
- Rhyu Im Joo; Soontaek Hong; Kim, Hyun
- Issue Date
- Mar-2011
- Publisher
- 한국현미경학회
- Citation
- 한국현미경학회지, v.41, no.1, pp.69 - 73
- Journal Title
- 한국현미경학회지
- Volume
- 41
- Number
- 1
- Start Page
- 69
- End Page
- 73
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/84633
- ISSN
- 12256773
- Abstract
- High-voltage electron microscope (HVEM) has
higher resolution and penetration power than conventional transmission
electron microscope that could be load thick specimen.
Some researchers have taken this advantage of HVEM to explore
3-dimensional configuration of the biological structures including
tissue and cells. Whole mount preparations has been employed to
study some cell lines and primary culture cells. In this study, we
would like to introduce useful whole mount preparation method
for neuronal studies.
The plastic coverslips were punched, covered by formvar membrane
and coated with carbon. The neurons obtained embryonic
18 rat hippocampus were seeded on the prepared cover slip. The
coverslips were fixed, dried in freeze drier and kept in a descicator
until HVEM observation.
We could observe detailed neuronal structures such as soma, dendrite
and spine under HVEM without conventional thin section
and heavy metal stain. The anaglyphic image based on stereo
paired image (-8??, +8??) provides three dimensional perception
of the neuronal dendrites and their spines.
This method could be applied to sophisticated analysis of dendritic
spine under the various experimental conditions.
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