Molecular characterization of a bactericidal permeability-increasing protein/lipopolysaccharide-binding protein from black rockfish (Sebastes schlegelii): Deciphering its putative antibacterial role
DC Field | Value | Language |
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dc.contributor.author | Lee, Seongdo | - |
dc.contributor.author | Elvitigala, Don Anushka Sandaruwan | - |
dc.contributor.author | Lee, Sukkyoung | - |
dc.contributor.author | Kim, Hyun Chul | - |
dc.contributor.author | Park, Hae-Chul | - |
dc.contributor.author | Lee, Jehee | - |
dc.date.accessioned | 2021-09-03T10:20:42Z | - |
dc.date.available | 2021-09-03T10:20:42Z | - |
dc.date.created | 2021-06-16 | - |
dc.date.issued | 2017-02 | - |
dc.identifier.issn | 0145-305X | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/84753 | - |
dc.description.abstract | Bactericidal permeability-increasing protein (BPD/lipopolysaccharide (LPS) binding proteins (LBPs) are well-known proteins that play an indispensable role in host antimicrobial defense. Herein, we report a homolog of BPI/LBP from black rockfish (Sebastes schlegelii) (designated as RfBPI/LBP) and characterize its structural and functional features at the molecular level. We identified the putative complete open reading frame (1422 bp) of RJLBP that encodes a 474 amino acid protein with a predicted molecular mass of similar to 51.5 kDa. The primary protein sequence of Rfl3PI/LBP contains domain features of BPI/LBP family proteins and shares significant sequence consistency with its homologs. Our phylogenetic analysis clearly demonstrated the vertebrate ancestral origin of RfBPI/LBP, further reinforcing its evolutionary relationship with teleostean homologs. Recombinant RfBPI/LBP demonstrated in vitro LPS-binding activity and antibacterial activity against Escherichia coli, but not against Streptococcus iniae. Moreover, RfBPI/LBP exhibited temporal transcriptional activation against pathogens and pathogen-associated molecular patterns. Collectively, our findings suggest that RfBPI/LBP plays an essential role in host antimicrobial defense, plausibly through selective eradication of invading bacteria. (C) 2016 Elsevier Ltd. All rights reserved. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | ELSEVIER SCI LTD | - |
dc.subject | LIPOPOLYSACCHARIDE (LPS)-BINDING PROTEIN | - |
dc.subject | EXPRESSION ANALYSIS | - |
dc.subject | BPI/LBP CDNA | - |
dc.subject | CLONING | - |
dc.subject | INNATE | - |
dc.subject | BPI | - |
dc.subject | FAMILY | - |
dc.subject | LPS | - |
dc.subject | COMPLEXES | - |
dc.subject | ENDOTOXIN | - |
dc.title | Molecular characterization of a bactericidal permeability-increasing protein/lipopolysaccharide-binding protein from black rockfish (Sebastes schlegelii): Deciphering its putative antibacterial role | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Park, Hae-Chul | - |
dc.identifier.doi | 10.1016/j.dci.2016.09.011 | - |
dc.identifier.scopusid | 2-s2.0-85000405876 | - |
dc.identifier.wosid | 000390071200026 | - |
dc.identifier.bibliographicCitation | DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY, v.67, pp.266 - 275 | - |
dc.relation.isPartOf | DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY | - |
dc.citation.title | DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY | - |
dc.citation.volume | 67 | - |
dc.citation.startPage | 266 | - |
dc.citation.endPage | 275 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Fisheries | - |
dc.relation.journalResearchArea | Immunology | - |
dc.relation.journalResearchArea | Veterinary Sciences | - |
dc.relation.journalResearchArea | Zoology | - |
dc.relation.journalWebOfScienceCategory | Fisheries | - |
dc.relation.journalWebOfScienceCategory | Immunology | - |
dc.relation.journalWebOfScienceCategory | Veterinary Sciences | - |
dc.relation.journalWebOfScienceCategory | Zoology | - |
dc.subject.keywordPlus | LIPOPOLYSACCHARIDE (LPS)-BINDING PROTEIN | - |
dc.subject.keywordPlus | EXPRESSION ANALYSIS | - |
dc.subject.keywordPlus | BPI/LBP CDNA | - |
dc.subject.keywordPlus | CLONING | - |
dc.subject.keywordPlus | INNATE | - |
dc.subject.keywordPlus | BPI | - |
dc.subject.keywordPlus | FAMILY | - |
dc.subject.keywordPlus | LPS | - |
dc.subject.keywordPlus | COMPLEXES | - |
dc.subject.keywordPlus | ENDOTOXIN | - |
dc.subject.keywordAuthor | LBP/BPI | - |
dc.subject.keywordAuthor | Black rockfish | - |
dc.subject.keywordAuthor | Antibacterial activity | - |
dc.subject.keywordAuthor | Immune stimulation | - |
dc.subject.keywordAuthor | Transcriptional regulation | - |
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