Molecular characterization of a bactericidal permeability-increasing protein/lipopolysaccharide-binding protein from black rockfish (Sebastes schlegelii): Deciphering its putative antibacterial role
- Authors
- Lee, Seongdo; Elvitigala, Don Anushka Sandaruwan; Lee, Sukkyoung; Kim, Hyun Chul; Park, Hae-Chul; Lee, Jehee
- Issue Date
- 2월-2017
- Publisher
- ELSEVIER SCI LTD
- Keywords
- LBP/BPI; Black rockfish; Antibacterial activity; Immune stimulation; Transcriptional regulation
- Citation
- DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY, v.67, pp.266 - 275
- Indexed
- SCIE
SCOPUS
- Journal Title
- DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
- Volume
- 67
- Start Page
- 266
- End Page
- 275
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/84753
- DOI
- 10.1016/j.dci.2016.09.011
- ISSN
- 0145-305X
- Abstract
- Bactericidal permeability-increasing protein (BPD/lipopolysaccharide (LPS) binding proteins (LBPs) are well-known proteins that play an indispensable role in host antimicrobial defense. Herein, we report a homolog of BPI/LBP from black rockfish (Sebastes schlegelii) (designated as RfBPI/LBP) and characterize its structural and functional features at the molecular level. We identified the putative complete open reading frame (1422 bp) of RJLBP that encodes a 474 amino acid protein with a predicted molecular mass of similar to 51.5 kDa. The primary protein sequence of Rfl3PI/LBP contains domain features of BPI/LBP family proteins and shares significant sequence consistency with its homologs. Our phylogenetic analysis clearly demonstrated the vertebrate ancestral origin of RfBPI/LBP, further reinforcing its evolutionary relationship with teleostean homologs. Recombinant RfBPI/LBP demonstrated in vitro LPS-binding activity and antibacterial activity against Escherichia coli, but not against Streptococcus iniae. Moreover, RfBPI/LBP exhibited temporal transcriptional activation against pathogens and pathogen-associated molecular patterns. Collectively, our findings suggest that RfBPI/LBP plays an essential role in host antimicrobial defense, plausibly through selective eradication of invading bacteria. (C) 2016 Elsevier Ltd. All rights reserved.
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