Generation of Retinal Progenitor Cells from Human Induced Pluripotent Stem Cell-Derived Spherical Neural Mass
DC Field | Value | Language |
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dc.contributor.author | Yun, Cheolmin | - |
dc.contributor.author | Oh, Jaeryung | - |
dc.contributor.author | Lee, Boram | - |
dc.contributor.author | Lee, Ja-Myong | - |
dc.contributor.author | Ariunaa, Togloom | - |
dc.contributor.author | Huh, Kuhl | - |
dc.date.accessioned | 2021-09-03T10:37:02Z | - |
dc.date.available | 2021-09-03T10:37:02Z | - |
dc.date.created | 2021-06-16 | - |
dc.date.issued | 2017-02 | - |
dc.identifier.issn | 1738-2696 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/84838 | - |
dc.description.abstract | Spherical neural mass (SNM) is a mass of neural precursors that have been used to generate neuronal cells with advantages of long-term passaging capability with high yield, easy storage, and thawing. In this study, we differentiated neural retinal progenitor cells (RPCs) from human induced pluripotent stem cells (hiPSC)-derived SNMs. RPCs were differentiated from SNMs with a noggin/fibroblast growth factor-basic/Dickkopf-1/Insulin-like growth factor-1/fibroblast growth factor-9 protocol for three weeks. Human RPCs expressed eye field markers (Paired box 6) and early neural retinal markers (Ceh-10 homeodomain containing homolog), but did not photoreceptor marker (Opsin 1 short-wave-sensitive). Reverse transcription polymerase chain reaction revealed that early neural retinal markers (Mammalian achaete-scute complex homolog 1, mouse atonal homolog 5, neurogenic differentiation 1) and retinal fate markers (brain-specific homeobox/POU domain transcription factor 3B and recoverin) were upregulated, while the marker of retinal pigment epithelium (microphthalmia-associated transcription factor) only showed slight upregulation. Human RPCs were transplanted into mouse (adult 8 weeks old C57BL/6) retina. Cells transplanted into the mouse retina matured and expressed markers of mature retinal cells (Opsin 1 short-wave-sensitive) and human nuclei on immunohistochemistry three months after transplantation. Development of RPCs using SNMs may offer a fast and useful method for neural retinal cell differentiation. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | KOREAN TISSUE ENGINEERING REGENERATIVE MEDICINE SOC | - |
dc.subject | PHOTORECEPTOR PRECURSORS | - |
dc.subject | MACULAR DEGENERATION | - |
dc.subject | DIFFERENTIATION | - |
dc.subject | INTEGRATION | - |
dc.subject | TRANSPLANTATION | - |
dc.subject | EYE | - |
dc.subject | REGENERATION | - |
dc.subject | EXPRESSION | - |
dc.subject | NEURONS | - |
dc.subject | MATURE | - |
dc.title | Generation of Retinal Progenitor Cells from Human Induced Pluripotent Stem Cell-Derived Spherical Neural Mass | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Oh, Jaeryung | - |
dc.identifier.doi | 10.1007/s13770-016-0021-2 | - |
dc.identifier.scopusid | 2-s2.0-85011924056 | - |
dc.identifier.wosid | 000394174200004 | - |
dc.identifier.bibliographicCitation | TISSUE ENGINEERING AND REGENERATIVE MEDICINE, v.14, no.1, pp.39 - 47 | - |
dc.relation.isPartOf | TISSUE ENGINEERING AND REGENERATIVE MEDICINE | - |
dc.citation.title | TISSUE ENGINEERING AND REGENERATIVE MEDICINE | - |
dc.citation.volume | 14 | - |
dc.citation.number | 1 | - |
dc.citation.startPage | 39 | - |
dc.citation.endPage | 47 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.identifier.kciid | ART002195270 | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.description.journalRegisteredClass | kci | - |
dc.relation.journalResearchArea | Cell Biology | - |
dc.relation.journalResearchArea | Engineering | - |
dc.relation.journalWebOfScienceCategory | Cell & Tissue Engineering | - |
dc.relation.journalWebOfScienceCategory | Engineering, Biomedical | - |
dc.subject.keywordPlus | PHOTORECEPTOR PRECURSORS | - |
dc.subject.keywordPlus | MACULAR DEGENERATION | - |
dc.subject.keywordPlus | DIFFERENTIATION | - |
dc.subject.keywordPlus | INTEGRATION | - |
dc.subject.keywordPlus | TRANSPLANTATION | - |
dc.subject.keywordPlus | EYE | - |
dc.subject.keywordPlus | REGENERATION | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | NEURONS | - |
dc.subject.keywordPlus | MATURE | - |
dc.subject.keywordAuthor | Human induced pluripotent stem cells | - |
dc.subject.keywordAuthor | Retinal photoreceptor | - |
dc.subject.keywordAuthor | Retinal progenitor cell | - |
dc.subject.keywordAuthor | Spherical neural mass | - |
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