Detailed Information

Cited 0 time in webofscience Cited 0 time in scopus
Metadata Downloads

Generation of Retinal Progenitor Cells from Human Induced Pluripotent Stem Cell-Derived Spherical Neural Mass

Authors
Yun, CheolminOh, JaeryungLee, BoramLee, Ja-MyongAriunaa, TogloomHuh, Kuhl
Issue Date
2월-2017
Publisher
KOREAN TISSUE ENGINEERING REGENERATIVE MEDICINE SOC
Keywords
Human induced pluripotent stem cells; Retinal photoreceptor; Retinal progenitor cell; Spherical neural mass
Citation
TISSUE ENGINEERING AND REGENERATIVE MEDICINE, v.14, no.1, pp.39 - 47
Indexed
SCIE
SCOPUS
KCI
Journal Title
TISSUE ENGINEERING AND REGENERATIVE MEDICINE
Volume
14
Number
1
Start Page
39
End Page
47
URI
https://scholar.korea.ac.kr/handle/2021.sw.korea/84838
DOI
10.1007/s13770-016-0021-2
ISSN
1738-2696
Abstract
Spherical neural mass (SNM) is a mass of neural precursors that have been used to generate neuronal cells with advantages of long-term passaging capability with high yield, easy storage, and thawing. In this study, we differentiated neural retinal progenitor cells (RPCs) from human induced pluripotent stem cells (hiPSC)-derived SNMs. RPCs were differentiated from SNMs with a noggin/fibroblast growth factor-basic/Dickkopf-1/Insulin-like growth factor-1/fibroblast growth factor-9 protocol for three weeks. Human RPCs expressed eye field markers (Paired box 6) and early neural retinal markers (Ceh-10 homeodomain containing homolog), but did not photoreceptor marker (Opsin 1 short-wave-sensitive). Reverse transcription polymerase chain reaction revealed that early neural retinal markers (Mammalian achaete-scute complex homolog 1, mouse atonal homolog 5, neurogenic differentiation 1) and retinal fate markers (brain-specific homeobox/POU domain transcription factor 3B and recoverin) were upregulated, while the marker of retinal pigment epithelium (microphthalmia-associated transcription factor) only showed slight upregulation. Human RPCs were transplanted into mouse (adult 8 weeks old C57BL/6) retina. Cells transplanted into the mouse retina matured and expressed markers of mature retinal cells (Opsin 1 short-wave-sensitive) and human nuclei on immunohistochemistry three months after transplantation. Development of RPCs using SNMs may offer a fast and useful method for neural retinal cell differentiation.
Files in This Item
There are no files associated with this item.
Appears in
Collections
College of Medicine > Department of Medical Science > 1. Journal Articles

qrcode

Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.

Related Researcher

Researcher Oh, Jae Ryung photo

Oh, Jae Ryung
의과대학 (의학과)
Read more

Altmetrics

Total Views & Downloads

BROWSE