RanBPM inhibits BLT2-mediated IL-8 production and invasiveness in aggressive breast cancer cells
- Authors
- Wei, Jun-Dong; Jang, Jae-Hyun; Kim, Jae-Hong
- Issue Date
- 29-1월-2017
- Publisher
- ACADEMIC PRESS INC ELSEVIER SCIENCE
- Keywords
- Breast cancer; BLT2; RanBPM; Invasiveness; IL-8
- Citation
- BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, v.483, no.1, pp.305 - 311
- Indexed
- SCIE
SCOPUS
- Journal Title
- BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
- Volume
- 483
- Number
- 1
- Start Page
- 305
- End Page
- 311
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/84864
- DOI
- 10.1016/j.bbrc.2016.12.147
- ISSN
- 0006-291X
- Abstract
- RanBPM is a scaffolding protein that regulates several cellular processes by interacting with various proteins. Previously, we reported that RanBPM acts as a negative regulator of BLT2, a low-affinity leukotriene B4 receptor; thus, it interferes with BLT2-mediated cell motility. In the present study, we observed that the expression levels of RanBPM were markedly reduced in the highly aggressive MDAMB- 435 and MDA-MB-231 human breast cancer cell lines compared with those in non-invasive MCF7 cells. Additionally, we found that the restoration of RanBPM levels suppressed the invasiveness of these aggressive breast cancer cells in a manner dependent on BLT2 activation. In contrast, the knockdown of endogenous RanBPM by shRNA strongly promoted invasiveness in non-invasive MCF-7 cells. We also observed that RanBPM suppressed the invasiveness of aggressive breast cancer cells by inhibiting BLT2mediated reactive oxygen species (ROS) generation and IL-8 production. Taken together, our results suggest that RanBPM acts as a negative regulator of BLT2, thus attenuating the invasiveness of aggressive breast cancer cells. (C) 2016 Elsevier Inc. All rights reserved.
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