Metabolic biomarkers for non-alcoholic fatty liver disease induced by high-fat diet: In vivo magnetic resonance spectroscopy of hyperpolarized [1-C-13] pyruvate
- Authors
- Moon, Chung-Man; Oh, Chang-Hyun; Ahn, Kyu-Youn; Yang, Jun-Seok; Kim, Jae-Young; Shin, Sang-Soo; Lim, Hyo-Soon; Heo, Sook-Hee; Seon, Hyun-Ju; Kim, Jin-Woong; Jeong, Gwang-Woo
- Issue Date
- 1-1월-2017
- Publisher
- ACADEMIC PRESS INC ELSEVIER SCIENCE
- Keywords
- Hyperpolarized C-13; Dynamic nuclear polarization (DNP); Non-alcoholic fatty liver disease (NAFLD); High-fat diet
- Citation
- BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, v.482, no.1, pp.112 - 119
- Indexed
- SCIE
SCOPUS
- Journal Title
- BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
- Volume
- 482
- Number
- 1
- Start Page
- 112
- End Page
- 119
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/84955
- DOI
- 10.1016/j.bbrc.2016.08.118
- ISSN
- 0006-291X
- Abstract
- Hyperpolarized C-13 magnetic resonance spectroscopy (MRS) to assess hepatic metabolism in nonalcoholic fatty liver disease (NAFLD) has not been reported. This study searched for cellular metabolism-based biomarkers for NAFLD induced by a high-fat diet (HFD) in rats. Also, correlations of the biomarkers with enzyme levels and histopathology were identified during a 6-week follow-up. Six rats were fed a control diet (CD) and seven rats were fed the HFD for 6 weeks. Hyperpolarized 13C dynamic MRS was performed on rat liver following an injection of hyperpolarized [1-C-13] pyruvate. Compared with CD-fed rats, HFD-fed rats showed significant increases in the levels of serum alanine aminotransferase and low-density lipoprotein cholesterol at weeks 4 and 6 of follow-up. After the 6 week HFD, the ratios of [1-C-13] alanine/pyruvate and [1-C-13] lactate/pyruvate were significantly increased, as were the levels of alanine aminotransferase and lactate dehydrogenase, which are potentially associated with hepatosteatosis. The results implicate [1-C-13] alanine and [1-C-13] lactate as potentially useful noninvasive biomarkers of hepatosteatosis occurring in NAFLD. (C) 2016 Published by Elsevier Inc.
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