TEMPO-Assisted Free Radical-Initiated Peptide Sequencing Mass Spectrometry (FRIPS MS) in Q-TOF and Orbitrap Mass Spectrometers: Single-Step Peptide Backbone Dissociations in Positive Ion Mode
DC Field | Value | Language |
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dc.contributor.author | Jang, Inae | - |
dc.contributor.author | Lee, Sun Young | - |
dc.contributor.author | Hwangbo, Song | - |
dc.contributor.author | Kang, Dukjin | - |
dc.contributor.author | Lee, Hookeun | - |
dc.contributor.author | Kim, Hugh I. | - |
dc.contributor.author | Moon, Bongjin | - |
dc.contributor.author | Oh, Han Bin | - |
dc.date.accessioned | 2021-09-03T11:30:18Z | - |
dc.date.available | 2021-09-03T11:30:18Z | - |
dc.date.created | 2021-06-16 | - |
dc.date.issued | 2017-01 | - |
dc.identifier.issn | 1044-0305 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/85055 | - |
dc.description.abstract | The present study demonstrates that one-step peptide backbone fragmentations can be achieved using the TEMPO [2-(2,2,6,6-tetramethyl piperidine-1-oxyl)]-assisted free radical-initiated peptide sequencing (FRIPS) mass spectrometry in a hybrid quadrupole time-of-flight (Q-TOF) mass spectrometer and a Q-Exactive Orbitrap instrument in positive ion mode, in contrast to two-step peptide fragmentation in an ion-trap mass spectrometer (reference Anal. Chem. 85, 7044-7051 (30)). In the hybrid Q-TOF and Q-Exactive instruments, higher collisional energies can be applied to the target peptides, compared with the low collisional energies applied by the ion-trap instrument. The higher energy deposition and the additional multiple collisions in the collision cell in both instruments appear to result in one-step peptide backbone dissociations in positive ion mode. This new finding clearly demonstrates that the TEMPO-assisted FRIPS approach is a very useful tool in peptide mass spectrometry research. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | SPRINGER | - |
dc.subject | SIDE-CHAIN CLEAVAGE | - |
dc.subject | GAS-PHASE | - |
dc.subject | DIRECTED DISSOCIATION | - |
dc.subject | FRAGMENTATION | - |
dc.subject | CATIONS | - |
dc.subject | PHOTODISSOCIATION | - |
dc.title | TEMPO-Assisted Free Radical-Initiated Peptide Sequencing Mass Spectrometry (FRIPS MS) in Q-TOF and Orbitrap Mass Spectrometers: Single-Step Peptide Backbone Dissociations in Positive Ion Mode | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Kim, Hugh I. | - |
dc.identifier.doi | 10.1007/s13361-016-1508-8 | - |
dc.identifier.scopusid | 2-s2.0-85006412429 | - |
dc.identifier.wosid | 000391433100018 | - |
dc.identifier.bibliographicCitation | JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY, v.28, no.1, pp.154 - 163 | - |
dc.relation.isPartOf | JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY | - |
dc.citation.title | JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY | - |
dc.citation.volume | 28 | - |
dc.citation.number | 1 | - |
dc.citation.startPage | 154 | - |
dc.citation.endPage | 163 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.relation.journalResearchArea | Spectroscopy | - |
dc.relation.journalWebOfScienceCategory | Biochemical Research Methods | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Analytical | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Physical | - |
dc.relation.journalWebOfScienceCategory | Spectroscopy | - |
dc.subject.keywordPlus | SIDE-CHAIN CLEAVAGE | - |
dc.subject.keywordPlus | GAS-PHASE | - |
dc.subject.keywordPlus | DIRECTED DISSOCIATION | - |
dc.subject.keywordPlus | FRAGMENTATION | - |
dc.subject.keywordPlus | CATIONS | - |
dc.subject.keywordPlus | PHOTODISSOCIATION | - |
dc.subject.keywordAuthor | Free radical-initiated peptide sequencing (FRIPS) | - |
dc.subject.keywordAuthor | TEMPO | - |
dc.subject.keywordAuthor | Radical-based fragmentations | - |
dc.subject.keywordAuthor | Q-TOF | - |
dc.subject.keywordAuthor | Orbitrap | - |
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