TEMPO-Assisted Free Radical-Initiated Peptide Sequencing Mass Spectrometry (FRIPS MS) in Q-TOF and Orbitrap Mass Spectrometers: Single-Step Peptide Backbone Dissociations in Positive Ion Mode
- Authors
- Jang, Inae; Lee, Sun Young; Hwangbo, Song; Kang, Dukjin; Lee, Hookeun; Kim, Hugh I.; Moon, Bongjin; Oh, Han Bin
- Issue Date
- 1월-2017
- Publisher
- SPRINGER
- Keywords
- Free radical-initiated peptide sequencing (FRIPS); TEMPO; Radical-based fragmentations; Q-TOF; Orbitrap
- Citation
- JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY, v.28, no.1, pp.154 - 163
- Indexed
- SCIE
SCOPUS
- Journal Title
- JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY
- Volume
- 28
- Number
- 1
- Start Page
- 154
- End Page
- 163
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/85055
- DOI
- 10.1007/s13361-016-1508-8
- ISSN
- 1044-0305
- Abstract
- The present study demonstrates that one-step peptide backbone fragmentations can be achieved using the TEMPO [2-(2,2,6,6-tetramethyl piperidine-1-oxyl)]-assisted free radical-initiated peptide sequencing (FRIPS) mass spectrometry in a hybrid quadrupole time-of-flight (Q-TOF) mass spectrometer and a Q-Exactive Orbitrap instrument in positive ion mode, in contrast to two-step peptide fragmentation in an ion-trap mass spectrometer (reference Anal. Chem. 85, 7044-7051 (30)). In the hybrid Q-TOF and Q-Exactive instruments, higher collisional energies can be applied to the target peptides, compared with the low collisional energies applied by the ion-trap instrument. The higher energy deposition and the additional multiple collisions in the collision cell in both instruments appear to result in one-step peptide backbone dissociations in positive ion mode. This new finding clearly demonstrates that the TEMPO-assisted FRIPS approach is a very useful tool in peptide mass spectrometry research.
- Files in This Item
- There are no files associated with this item.
- Appears in
Collections - College of Science > Department of Chemistry > 1. Journal Articles
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.