Visualization of Macrophage Recruitment to Inflammation Lesions using Highly Sensitive and Stable Radionuclide-Embedded Gold Nanoparticles as a Nuclear Bio-Imaging Platform
- Authors
- Lee, Sang Bong; Lee, Ho Won; Singh, Thoudam Debraj; Li, Yinghua; Kim, Sang Kyoon; Cho, Sung Jin; Lee, Sang-Woo; Jeong, Shin Young; Ahn, Byeong-Cheol; Choi, Sangil; Lee, In-Kyu; Lim, Dong-Kwon; Lee, Jaetae; Jeon, Yong Hyun
- Issue Date
- 2017
- Publisher
- IVYSPRING INT PUBL
- Keywords
- gold nanoparticles; nuclear bio-imaging platform; macrophage migration; acute inflammation
- Citation
- THERANOSTICS, v.7, no.4, pp.926 - 934
- Indexed
- SCIE
SCOPUS
- Journal Title
- THERANOSTICS
- Volume
- 7
- Number
- 4
- Start Page
- 926
- End Page
- 934
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/86428
- DOI
- 10.7150/thno.17131
- ISSN
- 1838-7640
- Abstract
- Reliable and sensitive imaging tools are required to track macrophage migration and provide a better understating of their biological roles in various diseases. Here, we demonstrate the possibility of radioactive iodide-embedded gold nanoparticles (RIe-AuNPs) as a cell tracker for nuclear medicine imaging. To demonstrate this utility, we monitored macrophage migration to carrageenan-induced sites of acute inflammation in living subjects and visualized the effects of anti-inflammatory agents on this process. Macrophage labeling with RIe-AuNPs did not alter their biological functions such as cell proliferation, phenotype marker expression, or phagocytic activity. In vivo imaging with positron-emission tomography revealed the migration of labeled macrophages to carrageenan-induced inflammation lesions 3 h after transfer, with highest recruitment at 6 h and a slight decline of radioactive signal at 24 h; these findings were highly consistent with the data of a bio-distribution study. Treatment with dexamethasone (an anti-inflammation drug) or GSK5182 (an ERR. inverse agonist) hindered macrophage recruitment to the inflamed sites. Our findings suggest that a cell tracking strategy utilizing RIe-AuNPs will likely be highly useful in research related to macrophage-related disease and cell-based therapies.
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Collections - Graduate School > KU-KIST Graduate School of Converging Science and Technology > 1. Journal Articles
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